Salvia miltiorrhiza reduces experimentally-induced hepatic fibrosis in rats
Salvia miltiorrhiza reduces experimentally-induced hepatic fibrosis in rats
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DOI：
10.1016/s0168-8278(98)80257-2
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发表时间：
1998-11-01
影响因子：
25.7
通讯作者：
Tan, CEL
中科院分区：
文献类型：
--
作者：
Wasser, S;Ho, JMS;Tan, CEL
背景/目的：肝纤维化是肝实质和胆道系统损伤的结果。我们研究了中药丹参的作用。方法：雄性Wistar大鼠慢性四氯化碳灌胃10周，诱导肝纤维化。四氯化碳处理大鼠随机分为三组：不给药组，从四氯化碳治疗开始12周服用鼠尾草，10周疗程结束后服用鼠尾草2周。正常对照组：既不服用四氯化碳，也不服用鼠尾草，只服用鼠尾草12周。对肝组织进行组织学分级，逆转录聚合酶链反应分析肝纤维化相关基因转化生长因子- β 1、细胞外基质成分前胶原I、III、组织金属蛋白酶-1和基质金属蛋白酶-13的转录，与甘油醛-3-磷酸脱氢酶转录归一化并进行统计学分析。组织学评价表明，丹参能逆转四氯化碳处理引起的纤维化。与疾病对照组相比，用中药治疗的大鼠的转化生长因子- β 1、前胶原I和III和金属蛋白酶-1组织抑制剂转录物水平降低，基质金属蛋白酶-13转录物水平升高。结论：丹参对四氯化碳诱导的大鼠肝纤维化有明显的抑制作用。
Background/Aims: Hepatic fibrosis occurs as a result of injury to the liver parenchyma and biliary system. We have studied the effect of the traditional Chinese medicinal herb, Salvia miltiorrhiza? in an experimental model of hepatic fibrosis and evaluated its effect on various paradigms involved in hepatic fibrosis,Methods: Liver fibrosis was induced in male Wistar rats by chronic administration of carbon tetrachloride for 10 weeks. The carbon tetrachloride-treated rats mere randomly assigned to three groups: no treatment, Salvia for 12 weeks from the onset of carbon tetrachloride treatment, and Salvia for 2 weeks after the completion of the 10-week course, The normal control groups in the study were: neither carbon tetrachloride nor Salvia, and Salvia only for 12 weeks. The livers were graded histologically and analyzed by reverse transcription-polymerase chain reaction for the transcription of genes involved in liver fibrosis, namely transforming growth factor-beta 1 and the extracellular matrix components procollagens I and III, tissue inhibitor of metalloproteinase-1 and matrix metalloproteinase-13, The transcripts were normalized against that of glyceraldehyde-3-phosphate dehydrogenase and analyzed statistically,Results: The histological evaluation showed that Salvia could reverse the fibrosis caused by carbon tetrachloride treatment. Rats treated with the herb had reduced levels of transforming growth factor-beta 1, procollagens I and III and tissue inhibitor of metalloproteinase-1 transcripts and an increased level of matrix metalloproteinase-13 transcript, when compared to the disease control.Conclusion: Salvia miltiorrhiza, a cheap and widely available herb significantly reduces carbon tetrachloride-induced hepatic fibrosis in rats.