High diversity of HIV-1 subtype F strains in Central Africa.
High diversity of HIV-1 subtype F strains in Central Africa.
复制标题
中部非洲 HIV-1 F 亚型毒株的高度多样性。
作者：
K. Triques;A. Bourgeois;S. Saragosti;N. Vidal;E. Mpoudi;N. Nzilambi;C. Apetrei;M. Ekwalanga;E. Delaporte;M. Peeters
在本文中，我们研究了非洲 HIV-1 F 亚型毒株的变异性。对于主要起源于中非的 11 种病毒，基因组的不同部分进行了遗传特征分析。对所有菌株的包膜基因的 V3-V5 区域进行了测序，并且对 7 个菌株的整个包膜基因进行了研究。对于 10 个菌株，gag 基因的 p24 区域也进行了测序。对于所研究的每个区域，确定了 F 亚型的三个亚组：F1、F2 和 F3。这三个亚组得到了高 bootstrap 值的支持，并且亚组内和亚组间 F 距离与已知亚型 A、B、C、D、E、G 和 H 获得的距离相当。在 F1 亚组中，一些非洲毒株与先前描述的来自巴西和罗马尼亚的毒株聚集在一起，表明这些国家的 HIV-1 流行病起源于非洲。对gag和包膜序列进行更详细的分析可以鉴定出四种重组病毒。我们的数据显示 F 亚型菌株之间存在高度多样性，表明所研究的区域中存在新的亚型。如果亚型之间存在生物学差异，那么明确定义这些亚型就很重要。我们的研究数据表明，需要明确识别 F 亚型内的不同亚组。
In this paper, we studied the variability of HIV-1 subtype F strains in Africa. For 11 viruses, mainly of Central African origin, different parts of the genome were genetically characterized. For all strains the V3-V5 region of the envelope gene was sequenced, and for 7 strains, the entire envelope gene was studied. For 10 strains, the p24 region of the gag gene was also sequenced. For each region studied, three subgroups in the F subtype were identified, F1, F2, and F3. These three subgroups were supported by high bootstrap values and the intra- and inter-subgroup F distances were comparable to those obtained for the known subtypes A, B, C, D, E, G, and H. In subgroup F1, some African strains clustered with previously described strains from Brazil and Romania, suggesting an African origin of the HIV-1 epidemic in these countries. A more detailed analysis of the gag and the envelope sequences allowed the identification of four recombinant viruses. Our data show a high diversity among subtype F strains, suggesting the presence of new subtypes in the regions studied. If biological differences exist among subtypes, it is important that these subtypes be well defined. The data from our study show that there is a need to clearly identify the different subgroups within the F subtype.
影响因子：
56.9
作者：
PRESTON, BD;POIESZ, BJ;LOEB, LA
通讯作者：
LOEB, LA
影响因子：
56.9
作者：
LARDER, BA;DARBY, G;RICHMAN, DD
通讯作者：
RICHMAN, DD