BIOCIDE SUSCEPTIBILITY AND INTRACELLULAR GLUTATHIONE IN ESCHERICHIA-COLI
BIOCIDE SUSCEPTIBILITY AND INTRACELLULAR GLUTATHIONE IN ESCHERICHIA-COLI
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DOI：
10.1007/bf01569672
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发表时间：
1993-12-01
期刊：
影响因子：
--
通讯作者：
LYMAN, RC
中科院分区：
文献类型：
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作者：
CHAPMAN, JS;DIEHL, MA;LYMAN, RC
研究了不同作用机制的杀菌剂对细胞内谷胱甘肽水平的生长和杀伤速度的抑制作用。缺乏细胞内谷胱甘肽的菌株对亲电杀菌剂敏感，但异噻唑酮杀菌剂除外。季铵化合物和自由基生成杀菌剂对生长的抑制作用不受细胞内谷胱甘肽水平的影响。杀死实验的速度表明，在对数和固定相培养谷胱甘肽缺乏菌株的甲醛杀死速度比其野生型亲本更快。在对数相培养中，谷胱甘肽水平对过氧化氢的杀伤速度没有影响，但在固定相培养中导致杀伤速度增加。谷胱甘肽缺乏菌株的固定相培养物被季铵杀菌剂杀死的速度比谷胱甘肽充满菌株慢。这些研究提供了杀菌剂的作用机制以及谷胱甘肽在确定杀菌剂敏感性方面的作用。
Inhibition of growth and speed of kill by biocides with different mechanisms of action was examined with respect to intracellular glutathione levels. A strain deficient in intracellular glutathione was hypersusceptible to electrophilic biocides, with the exception of an isothiazolone biocide. Growth inhibition by quaternary ammonium compounds and radical-generating biocides was unaffected by intracellular glutathione levels. Speed of kill experiments demonstrated a faster rate of killing by formaldehyde in both log and stationary phase cultures of the glutathione-deficient strain as compared to its wild-type parent. Glutathione levels had no effect on the speed of kill by hydrogen peroxide in log phase cultures, but resulted in an increased rate of killing in stationary phase cultures. Stationary phase cultures of the glutathione-deficient strain were killed by a quaternary ammonium biocide at a slower rate than the glutathione-replete strain. These studies provide information about both the mechanism of action of biocides as well as the role of glutathione in determining microbicide susceptibility.