NEUTRALIZING EPITOPES OF AFRICAN HORSESICKNESS VIRUS SEROTYPE-4 ARE LOCATED ON VP2
NEUTRALIZING EPITOPES OF AFRICAN HORSESICKNESS VIRUS SEROTYPE-4 ARE LOCATED ON VP2
复制标题
DOI：
10.1006/viro.1993.1537
复制
发表时间：
1993-10-01
期刊：
影响因子：
3.7
通讯作者：
LAEGREID, WW
中科院分区：
文献类型：
--
作者：
BURRAGE, TG;TREVEJO, R;LAEGREID, WW
制备了一组针对非洲马蹄疫病毒血清型4 （AHSV/4）的单克隆抗体（mab）。其中三种单克隆抗体（SA6、OH3和ME11）能强烈中和同源病毒和异源4型分离物。单克隆抗体不能交叉中和AHS血清型1-3或5-9。单克隆抗体免疫沉淀了一个108 kDa的病毒蛋白，该蛋白与VP2共迁移。经SA6预处理后，经颅内注射100 LD50 AHSV/4的日龄小鼠的死亡率为71%，而OH3和ME11显著提高了攻毒动物的平均存活时间。本研究表明，AHS的中和表位位于VP2上，这些表位的抗体在新生小鼠模型中具有保护作用。
A panel of monoclonal antibodies (MAbs) was generated against African horsesickness virus serotype 4 (AHSV/4). Three of the MAbs (SA6, OH3, and ME11) strongly neutralized the homologous virus and a heterologous type 4 isolate. The MAbs did not cross-neutralize AHS serotypes 1-3 or 5-9. The MAbs immunoprecipitated a viral protein of 108 kDa which co-migrated with VP2. Pretreatment with SA6 prevented mortality of 71% of day-old mice after intracranial injection of 100 LD50 of AHSV/4, while OH3 and ME11 significantly increased the average survival time of challenged animals. This study demonstrates that neutralizing epitope(s) for AHS are located on VP2 and that antibodies to these epitope(s) are protective in a neonatal mouse model.