A combined binary interaction and phenotypic map of C. elegans cell polarity proteins.
A combined binary interaction and phenotypic map of C. elegans cell polarity proteins.
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DOI：
10.1038/ncb3300
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发表时间：
2016-03
影响因子：
21.3
通讯作者：
Boxem M
中科院分区：
文献类型：
--
作者：
Koorman T;Klompstra D;van der Voet M;Lemmens I;Ramalho JJ;Nieuwenhuize S;van den Heuvel S;Tavernier J;Nance J;Boxem M
细胞极性的建立是多细胞生物体发育以及细胞和组织功能发挥的重要过程。在这里，我们结合联合收割机大规模的蛋白质相互作用映射与系统的表型分析，研究网络的物理相互作用的基础上的极性建立和维护的线虫秀丽隐杆线虫。使用基于片段的酵母双杂交策略，我们确定了296种蛋白质之间的439种相互作用，以及介导这些相互作用的蛋白质区域。网络的表型分析导致鉴定出100个物理相互作用的蛋白质对，对于这些蛋白质对，RNAi介导的耗尽在相同的极性相关过程中引起缺陷。我们通过证明RhoGAP PAC-1和PAR-6之间的物理相互作用是C.线虫胚胎我们的网络代表了候选相互作用的宝贵资源，可用于进一步了解细胞极化。
The establishment of cell polarity is an essential process for the development of multicellular organisms and the functioning of cells and tissues. Here, we combine large-scale protein interaction mapping with systematic phenotypic profiling to study the network of physical interactions that underlies polarity establishment and maintenance in the nematode Caenorhabditis elegans. Using a fragment-based yeast two-hybrid strategy, we identified 439 interactions between 296 proteins, as well as the protein regions that mediate these interactions. Phenotypic profiling of the network resulted in the identification of 100 physically interacting protein pairs for which RNAi-mediated depletion caused a defect in the same polarity-related process. We demonstrate the predictive capabilities of the network by showing that the physical interaction between the RhoGAP PAC-1 and PAR-6 is required for radial polarization of the C. elegans embryo. Our network represents a valuable resource of candidate interactions that can be used to further our insight into cell polarization.