Exploring miRNAs involved in blue/UV-A light response in Brassica rapa reveals special regulatory mode during seedling development.
Exploring miRNAs involved in blue/UV-A light response in Brassica rapa reveals special regulatory mode during seedling development.
复制标题
探索白菜中参与蓝光/UV-A光响应的miRNA揭示了幼苗发育过程中的特殊调控模式
DOI：
10.1186/s12870-016-0799-z
复制
发表时间：
2016-05-10
影响因子：
5.3
通讯作者：
Xu Q
中科院分区：
文献类型：
--
作者：
Zhou B;Fan P;Li Y;Yan H;Xu Q
研究背景芜菁的生长发育和色素合成。拉帕湾津田，一种受欢迎的蔬菜作物，受光的影响。虽然microRNAs（miRNAs）在植物的代谢过程和非生物胁迫反应中具有重要的作用，无论是发挥作用的花青苷的生物合成和发育的津田幼苗暴露于light.Results17保守和226新的miRNAs不同至少2倍，在响应蓝色和UV-A光与黑暗处理后的水平相比。真实的时间PCR结果表明，BrmiR 159、BrmiRC 0191、BrmiRC 0460、BrmiRC 0323、BrmiRC 0418、BrmiRC 0005是蓝光诱导的;北方杂交结果表明，BrmiR 167在暗、蓝光和紫外光处理下的转录水平差异不显著。BrmiR 156和BrmiR 157以最大量（读段数）存在，并且在SPL基因家族中它们的8个推定靶标中，在蓝色或UV-A暴露后，仅SPL 9（Bra 004674）和SPL 15（Bra 003305）的表达增加。此外，miR 157引导的靶SPL 9 mRNA的切割（Bra 004674，Bra 016891）和SPL 15 mRNA（Bra003305，Bra 014599）发生在miR 157序列结合区5′端10或11个碱基处。鉴定了芜菁幼苗中诱导的光形态发生表型，为津田幼苗中蓝色和UV-A光响应性miRNA提供了新的见解，并提供了miRNA多个靶点及其在植物发育中不同作用的证据。
BackgroundGrowth, development, and pigment synthesis in Brassica rapa subsp. rapa cv. Tsuda, a popular vegetable crop, are influenced by light. Although microRNAs (miRNAs) have vital roles in the metabolic processes and abiotic stress responses of plants, whether miRNAs play a role in anthocyanin biosynthesis and development of Tsuda seedlings exposed to light is unknown.ResultsSeventeen conserved and 226 novel miRNAs differed at least 2-fold in response to blue and UV-A light compared with levels after a dark treatment. Real time PCR showed that BrmiR159, BrmiRC0191, BrmiRC0460, BrmiRC0323, BrmiRC0418, BrmiRC0005 were blue light-induced and northern blot revealed that the transcription level of BrmiR167 did not differ significantly among seedlings treated with dark, blue or UV-light. BrmiR156 and BrmiR157 were present in the greatest amount (number of reads) and among their 8 putative targets in the SPL gene family, only SPL9 (Bra004674) and SPL15 (Bra003305) increased in expression after blue or UV-A exposure. In addition, miR157-guided cleavage of target SPL9 mRNAs (Bra004674, Bra016891) and SPL15 mRNAs (Bra003305, Bra014599) took place 10 or 11 bases from the 5′ ends of the binding region in the miR157 sequence.ConclusionsA set of miRNAs and their targets involved in the regulation of the light-induced photomorphogenic phenotype in seedlings of Brassica rapa was identified, providing new insights into blue and UV-A light-responsive miRNAs in seedlings of Tsuda and evidence of multiple targets for the miRNAs and their diverse roles in plant development.