A simplified isolated perfused rat liver apparatus: characterization and measurement of extraction ratios of selected compounds.
A simplified isolated perfused rat liver apparatus: characterization and measurement of extraction ratios of selected compounds.
复制标题
简化的离体灌注大鼠肝脏装置：所选化合物提取率的表征和测量。
DOI：
10.1016/s0024-3205(00)00503-8
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发表时间：
2000
期刊：
影响因子：
6.1
通讯作者：
Mayersohn,M
中科院分区：
文献类型：
--
作者：
Sinha,V;Brendel,K;Mayersohn,M
A simplified isolated perfused rat liver (IPRL) preparation has been developed and evaluated. The liver is briefly perfused in situ prior to being placed into a 37 °C oven and suspended from a stand. This set-up takes about 5 min. A non-recirculatory or one-pass perfusion approach has been used. The performance of the apparatus was evaluated with use of three model compounds: antipyrine, lidocaine and ethanol. In addition, oxygen extraction was determined. The steady-state extraction ratio (ER) was determined for each compound (and oxygen) as a function of perfusate flow rate (15–35 ml min ) during sequential 45 min perfusion periods. Perfusion experiments lasted for up to 3 hr. The ERs (at 15 ml min ) of ethanol (0.65 ± 0.15), lidocaine (0.91 ± 0.01) and oxygen (0.65 ± 0.10) were dependent upon perfusate flow; whereas, antipyrine ER (0.07 ± 0.01) was independent of flow. The corresponding values for unbound intrinsic clearances (CLu,int) for antipyrine, ethanol, lidocaine and oxygen were: 1.6, 31.0, 158.0 and 27.5 ml min , respectively. These findings are consistent with the known hepatic ER values for those compounds reported in the literature.
