Lectin receptors in the cockroach neuromuscular system. II. Effects of denervation on muscle lectin receptors.
Lectin receptors in the cockroach neuromuscular system. II. Effects of denervation on muscle lectin receptors.
复制标题
蟑螂神经肌肉系统中的凝集素受体。
DOI：
10.1016/0006-8993(83)90026-4
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发表时间：
1983
期刊：
影响因子：
2.9
通讯作者：
Caldwell,RT
中科院分区：
文献类型：
--
作者：
Denburg,JL;Eastburn,JL;Caldwell,RT
在去神经后 1、2、3 和 5 周检查植物凝集素的荧光衍生物与蟑螂腿部 6 块臀部降压肌的结合。对凝集素与肌肉冰冻切片结合的组织化学分析表明，所有可检测到的结合均与肌纤维表面结合。此外，在去神经支配的一周内，那些处于神经支配状态的肌肉表面上具有不同量的α-半乳糖和/或α-N-乙酰半乳糖胺，现在都具有相同量的这些碳水化合物。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳对伴刀豆球蛋白 A 和麦芽凝集素糖蛋白受体进行生化分析表明，检测到的所有受体中约 17% 改变了去神经肌肉中的相对水平。这些变化是在每块肌肉中等量存在的受体以及以与已识别的运动神经元的神经支配相关的方式分布在 6 块肌肉中的受体中观察到的。然而，尽管这些变化减少了去神经 CDM 中的生化差异，但仍然可以通过 Con A 和 WGA 糖蛋白受体的性质来区分 6 块肌肉。去神经支配的肌肉仍然呈现出与再生运动神经元不同的生化细胞表面。
The binding of fluorescent derivatives of plant lectins to the 6 coxal depressor muscles in the leg of the cockroach was examined at 1, 2, 3 and 5 weeks after denervation. Histochemical analysis of the binding of the lectins to frozen sections of the muscles demonstrated that all detectable binding was to the surfaces of the muscle fibers. In addition, within one week of denervation those muscles which in the innervated state have different amounts ofd-galactose and/or α-N-acetylgalactosamine on their surfaces, now all have identical amounts of these carbohydrates. Biochemical analysis of concanavalin A and wheat germ agglutinin glycoprotein receptors by sodium dodecylsulphate polyacrylamide gel electrophoresis indicated that approximately 17% of all the receptors detected altered their relative levels in denervated muscles. These changes were observed in those receptors that were present in equal amounts in each of the muscles as well as in those receptors that were distributed among the 6 muscles in a manner that correlated with innervation by an identified motor neuron. However, in spite of these changes that reduced the biochemical differences in the denervated CDMs it was still possible to distinguish among the 6 muscles by the nature of the Con A and WGA glycoprotein receptors. Denervated muscles still present biochemically different cell surfaces to regenerating motor neurons.