Expression of carboxylesterase and lipase genes in rat liver cell-types
Expression of carboxylesterase and lipase genes in rat liver cell-types
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DOI：
10.1016/j.bbrc.2008.07.024
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发表时间：
2008-09-26
影响因子：
3.1
通讯作者：
Sanghani, Sonal P.
中科院分区：
文献类型：
--
作者：
Mello, Tommaso;Nakatsuka, Alice;Sanghani, Sonal P.
大约80%的维生素A储存在肝星状细胞中，并以视黄酯的形式储存在脂滴中。在低维生素A状态下或肝损伤后，星状细胞通过水解为视黄醇而耗尽储存的视黄酯。然而，在星状细胞中表达的视黄酯水解酶的身份是未知的。采用实时荧光定量PCR技术检测纯化肝细胞中羧酸酯酶和脂肪酶基因的表达。我们发现六个羧酸酯酶和肝脂肪酶基因在肝细胞中表达。脂肪甘油三酯脂肪酶在枯否细胞、星状细胞和内皮细胞中表达。在Kupffer细胞和星状细胞中检测到脂蛋白脂酶的表达。作为星状细胞活化的函数，脂肪甘油三酯脂酶的表达降低2倍，脂蛋白脂酶增加32倍，提示其可能在星状细胞活化过程中参与视黄醇酯的水解。爱思唯尔公司出版
Approximately 80% of the body vitamin A is stored in liver stellate cells with in the lipid droplets as retinyl esters. In low vitamin A status or after liver injury, stellate cells are depleted of the stored retinyl esters by their hydrolysis to retinol. However, the identity of retinyl ester hydrolase(s) expressed in stellate cells is unknown. The expression of carboxylesterase and lipase genes in purified liver cell-types was investigated by real-time PCR. We found that six carboxylesterase and hepatic lipase genes were expressed in hepatocytes. Adipose triglyceride lipase was expressed in Kupffer cells, stellate cells and endothelial cells. Lipoprotein lipase expression was detected in Kupffer cells and stellate cells, As a function of stellate cell activation, expression of adipose triglyceride lipase decreased by twofold and lipoprotein lipase increased by 32-fold Suggesting that it may play a role in retinol ester hydrolysis during stellate cell activation. Published by Elsevier Inc.