Effect of E1A and E1B viral proteins on the expression of a calcium ionophore-inducible gene and its promoter.
Effect of E1A and E1B viral proteins on the expression of a calcium ionophore-inducible gene and its promoter.
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E1A 和 E1B 病毒蛋白对钙离子载体诱导基因及其启动子表达的影响。
DOI：
10.1093/nar/14.12.4911
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发表时间：
1986
影响因子：
14.9
通讯作者：
Lee,AS
中科院分区：
文献类型：
--
作者：
Lin,AY;Lee,AS
腺病毒ElA产品已被证明对病毒和细胞基因具有正调控和负调控作用。为了进一步研究E1A产物对细胞基因及其启动子的影响，我们在腺病毒转化后表达了E1A和E1B产物的293细胞中检测了两种钙离子调控基因（p3C5和p4A3）的内源性转录水平。此外，我们比较了293和其他细胞系在诱导和非诱导条件下p3C5启动子和猿猴病毒（SV40）早期启动子的活性。我们的研究结果表明，293细胞中E1A和E1B产物的存在对p3C5和p4A3基因的内源性表达水平没有显著影响，也不会对p3C5启动子活性产生负面影响。我们得出结论：(1)病毒产物对细胞基因表达的活性是选择性的；组成性表达和易于诱导的基因不一定是E1A功能的一般靶标。(2)虽然p3C5启动子含有一个与SV40启动子和其他病毒增强子核心序列具有序列同源性的增强子样元件，但其活性不受ElA产物的抑制。
The adenovirus ElA products have been shown to confer both positive and negative regulations on viral as well as cellular genes. To investigate further the effect of the E1A products on cellular genes and their promoters, we measured the endogenous transcript levels of two calcium lonophore-regulated genes (p3C5 and p4A3) in 293 cells which expressed the E1A and E1B products as a result of adenovirus transformation. In addition, we compared the activities of the p3C5 promoter and the simian virus (SV40) early promoter under both induced and non-induced conditions in 293 and other cell lines. Our results indicate that the presence of the E1A and E1B products in 293 cells do not have any significant effect on the endogenous level of expression of both p3C5 and p4A3 genes, nor does it negatively affect the p3C5 promoter activity. We conclude that (1) the activity of the viral products on cellular gene expression is selective; genes that are constitutively expressed and are readily inducible are not necessarily general targets for the E1A functions and (2) while the p3C5 promoter contains an enhancer-like element which shares sequence homology with the SV40 promoter and other viral enhancer core sequences, its activity is not repressed by the ElA products.