Real-time particle tracking at 10,000 fps using optical fiber illumination
Real-time particle tracking at 10,000 fps using optical fiber illumination
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DOI：
10.1364/oe.18.022722
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发表时间：
2010-10-25
期刊：
影响因子：
3.8
通讯作者：
Keyser, Ulrich F.
中科院分区：
文献类型：
--
作者：
Otto, Oliver;Czerwinski, Fabian;Keyser, Ulrich F.
我们介绍了光纤照明的实时跟踪光学捕获的微米级粒子的微秒级的时间分辨率。我们的光源是一个高辐射汞弧灯和600 μ m的光纤短距离照明的样品池。粒子跟踪是用软件实现的互相关算法进行图像采集后，从CMOS相机。我们的图像数据表明，光纤照明的结果在一个信号-噪声比通常一个数量级高于标准的科勒照明。我们展示了一个单一的光学捕获胶体的位置确定高达10,000帧每秒超过小时。我们校准我们的光镊和比较结果与象限光电二极管测量。最后，我们通过计算Allan方差将我们的设置的位置精度确定为2 nm。我们的研究结果表明，无论是照明还是软件算法限制的速度实时粒子跟踪CMOS技术。(C)2010年美国光学学会
We introduce optical fiber illumination for real-time tracking of optically trapped micrometer-sized particles with microsecond time resolution. Our light source is a high-radiance mercury arc lamp and a 600 mu m optical fiber for short-distance illumination of the sample cell. Particle tracking is carried out with a software implemented cross-correlation algorithm following image acquisition from a CMOS camera. Our image data reveals that fiber illumination results in a signal-to-noise ratio usually one order of magnitude higher compared to standard Kohler illumination. We demonstrate position determination of a single optically trapped colloid with up to 10,000 frames per second over hours. We calibrate our optical tweezers and compare the results with quadrant photo diode measurements. Finally, we determine the positional accuracy of our setup to 2 nm by calculating the Allan variance. Our results show that neither illumination nor software algorithms limit the speed of real-time particle tracking with CMOS technology. (C) 2010 Optical Society of America