SOMATIC-CELL HYBRIDIZATION OF MOUSE MYELOMA CELLS
SOMATIC-CELL HYBRIDIZATION OF MOUSE MYELOMA CELLS
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DOI：
10.1016/0092-8674(76)90153-7
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发表时间：
1976-01-01
期刊：
影响因子：
64.5
通讯作者：
SCHARFF, MD
中科院分区：
文献类型：
--
作者：
MARGULIES, DH;KUEHL, WM;SCHARFF, MD
不同的小鼠骨髓瘤细胞系之间的体细胞杂交很容易利用现有技术的改进来分离。这些细胞的杂交性质是通过HAT[次黄嘌呤和胸腺嘧啶]或HAT-哇巴因选择性程序、它们的染色体数目和1例H-2表面抗原的表达来确定的。本文描述了三个杂交瘤细胞系：免疫球蛋白G2B，KAPPA。IgG2a，.KAPPA；一个IgG1，.KAPPA.IgG2b，KAPPA和IgG1，KAPPA倍。IGM，.lambda..所有杂交细胞均表达亲本类型的H链和L链，未观察到新的链。分子中含有母体H链和/或L链的二硫键混合物。对这些杂交种的亚克隆分析表明，免疫球蛋白的表达在长达13mo的时间内相当稳定。不再合成1条或1条以上亲本H链或L链的分段性克隆频繁出现。
Somatic cell hybrids between different mouse myeloma cell lines were readily isolated using modifications of existing techniques. The hybrid nature of these cells was established by HAT [hypoxanthine and thymidine] or HAT-ouabain selective procedures, their chromosome number and, in 1 case, H-2 surface antigen expression. Three hybrid cell lines are described: an IgG2b [immunoglobulin G2b], .KAPPA. .times. IgG2a, .KAPPA.; an IgG1, .KAPPA. .times. IgG2b, .KAPPA. and an IgG1, .KAPPA. .times. IgM, .lambda.. In all cases, both parental types of H and L chains are expressed in the hybrid cells and no new chains are observed. Molecules possessing disulfide-bonded mixtures of parental H and/or L chains are seen. Analysis of subclones of these hybrids indicates considerable stability in the expression of the Ig for up to 13 mo. Segregant clones no longer synthesizing 1 or more of the parental H or L chains arise frequently.