Multicolor "DiOlistic" labeling of the nervous system using lipophilic dye combinations
Multicolor "DiOlistic" labeling of the nervous system using lipophilic dye combinations
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DOI：
10.1016/s0896-6273(00)00031-3
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发表时间：
2000-08-01
期刊：
影响因子：
16.2
通讯作者：
Lichtman, JW
中科院分区：
文献类型：
--
作者：
Gan, WB;Grutzendler, J;Lichtman, JW
我们描述了一种用多种不同颜色快速标记神经系统中大量细胞的技术。通过用“基因枪”将亲脂染料包裹的颗粒输送到神经元制剂中，被颗粒接触到膜的单个神经元和神经胶质细胞被快速标记。使用每个颗粒都涂有各种亲脂染料的不同组合，复杂神经元网络中的许多细胞可以同时用各种颜色进行标记。这种方法在活材料上最有效，但也在以前固定的材料上贴标签。在活的材料中，标记的神经元继续显示正常的突触反应并经历树突重塑。因此，这项技术对于研究活的制剂中神经元回路的结构可塑性是有用的。此外，许多不同颜色的神经元的高尔基体标记为研究神经元的连接提供了一种新的方法。
We describe a technique for rapid labeling of a large number of cells in the nervous system with many different colors. By delivering lipophilic dye-coated particles to neuronal preparations with a "gene gun," individual neurons and glia whose membranes are contacted by the particles are quickly labeled. Using particles that are each coated with different combinations of various lipophilic dyes, many cells within a complex neuronal network can be simultaneously labeled with a wide variety of colors. This approach is most effective in living material but also labels previously fixed material. In living material, labeled neurons continue to show normal synaptic responses and undergo dendritic remodeling. This technique is thus useful for studying structural plasticity of neuronal circuits in living preparations. In addition, the Golgi-like labeling of neurons with many different colors provides a novel way to study neuronal connectivity.