Say no to DMSO: dimethylsulfoxide inactivates cisplatin, carboplatin, and other platinum complexes.
Say no to DMSO: dimethylsulfoxide inactivates cisplatin, carboplatin, and other platinum complexes.
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DOI：
10.1158/0008-5472.can-14-0247
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发表时间：
2014-07-15
期刊：
影响因子：
11.2
通讯作者：
Gottesman MM
中科院分区：
文献类型：
--
作者：
Hall MD;Telma KA;Chang KE;Lee TD;Madigan JP;Lloyd JR;Goldlust IS;Hoeschele JD;Gottesman MM
铂类药物顺铂、卡铂和奥沙利铂在临床上的使用率很高，因此在实验室中得到了广泛的研究。在这项研究中，我们查阅了文献，发现在著名的癌症杂志上有相当数量的研究(11-34%)使用溶解在二甲基亚砜(DMSO)中的顺铂。然而，将顺铂溶解在DMSO中进行实验室研究会导致配体移位并改变络合物的结构。我们检测了DMSO对包括顺铂、卡铂和奥沙利铂在内的铂化合物的影响，发现DMSO与这些化合物发生反应，抑制了它们的细胞毒性和启动细胞死亡的能力。这些结果使得很大一部分关于顺铂的文献无法解释。提高癌症生物界对这一重大问题的认识是至关重要的，我们就研究用铂类药物的适当溶剂化提出了建议。
The platinum drugs cisplatin, carboplatin and oxaliplatin are highly utilized in the clinic and as a consequence are extensively studied in the laboratory setting. In this study, we examined the literature and found a significant number of studies (11 - 34%) in prominent cancer journals utilizing cisplatin dissolved in dimethylsulfoxide (DMSO). However, dissolving cisplatin in DMSO for laboratory-based studies results in ligand displacement and changes the structure of the complex. We examined the effect of DMSO on platinum complexes, including cisplatin, carboplatin and oxaliplatin, finding that DMSO reacted with the complexes, inhibited their cytotoxicity and their ability to initiate cell death. These results render a substantial portion of the literature on cisplatin uninterpretable. Raising awareness of this significant issue in the cancer biology community is critical, and we make recommendations on appropriate solvation of platinum drugs for research.