Bioactive molecule Icariin inhibited proliferation while enhanced apoptosis and autophagy of rat airway smooth muscle cells in vitro.
Bioactive molecule Icariin inhibited proliferation while enhanced apoptosis and autophagy of rat airway smooth muscle cells in vitro.
复制标题
生物活性分子淫羊藿苷在体外抑制大鼠气道平滑肌细胞的增殖，同时增强其凋亡和自噬。
DOI：
10.1007/s10616-019-00348-9
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发表时间：
2019
期刊：
影响因子：
2.2
通讯作者：
Yao Dong
中科院分区：
文献类型：
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作者：
Yang Lihong;Xiao Bo;Hou Lixia;Zhou Guiming;Mo Biwen;Yao Dong
淫羊藿苷是从淫羊藿中提取的主要活性成分，参与细胞增殖、凋亡、自噬等细胞行为的调节。对于许多细胞类型，但淫羊藿苷对气道平滑肌细胞（ASMCs）的作用仍然未知。本研究旨在探讨淫羊藿苷对大鼠动脉平滑肌细胞增殖、凋亡和自噬的影响。CKK8法显示淫羊藿苷呈剂量-时间依赖性抑制大鼠ASMCs生长，流式细胞术显示淫羊藿苷干预ASMCs细胞周期，使S期缩短，G2期延长，cyclin E1和cyclinA1基因及蛋白表达降低。流式细胞术和TUNEL法检测细胞凋亡，结果表明淫羊藿苷促进ASMCs凋亡，并增强凋亡蛋白c-caspase-3的表达。淫羊藿苷诱导大鼠ASMCs自噬，并增强自噬标志物LC 3 II的表达。结论：淫羊藿苷抑制ASMCs增殖，促进ASMCs凋亡和自噬，可能在治疗哮喘气道重塑中发挥作用。
Icariin is the main active compound extracted from epimedium Flavonoids (EFs) and involved in regulation of cell behaviors (proliferation, apoptosis, and autophagy etc.) for many cell types, but the effect of Icariin on airway smooth muscle cells (ASMCs) is still unknown. The aim of the present study is to examine the role of Icariin on rat ASMCs proliferation, apoptosis and autophagy. CKK8 assay showed that Icariin inhibited rat ASMCs growth in dose-time-dependent manner, and the flow cytometry assay showed that the Icariin interfered with ASMCs cell cycle, when treated with Icariin, S phase shortened while G2 phase extended, cyclin E1 and cyclinA1 gene and protein expression decreased. Next apoptosis was detected, Flow cytometry and TdTmediated dUTP Nick-End Labeling (TUNEL) assay showed that Icariin promoted ASMCs apoptosis, and enhanced apoptosis protein cleaved-caspase-3 expression. Finally, it was found Icariin induced rat ASMCs autophagy, with enhancement expression of autophagy marker LC3 II. In conclusion, Icariin inhibited ASMCs proliferation while promoted apoptosis and autophagy, revealing its potential role in treatment of airway remodeling in asthma.