Microtubules and the endoplasmic reticulum are highly interdependent structures.
Microtubules and the endoplasmic reticulum are highly interdependent structures.
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DOI：
10.1083/jcb.103.4.1557
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发表时间：
1986-10
期刊：
影响因子：
--
通讯作者：
Fujiwara K
中科院分区：
文献类型：
--
作者：
Terasaki M;Chen LB;Fujiwara K
内质网（ER），微管，和中间丝的相互关系进行了研究，在薄，传播成纤维细胞，上皮细胞和血管内皮细胞培养的周边地区。我们结合荧光染料染色技术定位ER与免疫荧光定位微管或中间丝在同一个细胞。微管和ER在板状伪足中稀少，但中间丝通常完全不存在。这些关系表明，微管和ER推进到板状伪足之前，中间丝。我们观察到，微管和微管的ER有几乎相同的分布在板状伪足，新的扩展都在发生。我们用诺考达唑、低温或低渗休克扰动微管，观察其对内质网分布的影响。根据我们在未处理细胞中的观察和我们的微管扰动实验，我们得出结论，微管和ER在两个方面高度相互依赖：（a）在荧光显微镜的分辨率水平上，单个微管的聚合和单个ER微管的延伸一起发生，和（B）微管的解聚在短期内（15分钟）不破坏ER网络，但长时间的微管缺失（2h）导致ER网络向细胞中心缓慢收缩，表明在较长的时间段内，整个ER网络的延伸状态需要微管系统。
The interrelationships of the endoplasmic reticulum (ER), microtubules, and intermediate filaments were studied in the peripheral regions of thin, spread fibroblasts, epithelial, and vascular endothelial cells in culture. We combined a fluorescent dye staining technique to localize the ER with immunofluorescence to localize microtubules or intermediate filaments in the same cell. Microtubules and the ER are sparse in the lamellipodia, but intermediate filaments are usually completely absent. These relationships indicate that microtubules and the ER advance into the lamellipodia before intermediate filaments. We observed that microtubules and tubules of the ER have nearly identical distributions in lamellipodia, where new extensions of both are taking place. We perturbed microtubules by nocodazole, cold temperature, or hypotonic shock, and observed the effects on the ER distribution. On the basis of our observations in untreated cells and our experiments with microtubule perturbation, we conclude that microtubules and the ER are highly interdependent in two ways: (a) polymerization of individual microtubules and extension of individual ER tubules occur together at the level of resolution of the fluorescence microscope, and (b) depolymerization of microtubules does not disrupt the ER network in the short term (15 min), but prolonged absence of microtubules (2 h) leads to a slow retraction of the ER network towards the cell center, indicating that over longer periods of time, the extended state of the entire ER network requires the microtubule system.