Cytosolic free calcium and cell spreading decrease in fibroblasts from aged and Alzheimer donors.
Cytosolic free calcium and cell spreading decrease in fibroblasts from aged and Alzheimer donors.
复制标题
老年和阿尔茨海默病捐献者的成纤维细胞中胞质游离钙和细胞扩散减少。
DOI：
10.1073/pnas.83.20.7999
复制
发表时间：
1986
影响因子：
11.1
通讯作者：
Goldman,JE
中科院分区：
文献类型：
--
作者：
Peterson,C;Ratan,RR;Shelanski,ML;Goldman,JE
衰老和阿尔茨海默病导致钙稳态的改变。用钙敏感的荧光染料quin-2和fura-2测定了衰老和阿尔茨海默病过程中培养的皮肤成纤维细胞胞浆游离钙的浓度。与年龄匹配的对照组相比，阿尔茨海默病供体显示出70%的下降（P小于0.001），与来自年轻成年供体的细胞相比，下降了81%（P小于0.001）。这种减少quin-2-钙荧光似乎不是由于重金属或细胞内pH值的改变淬灭。类似的减少，观察到与Fura-2的游离胞浆钙。此外，来自老年和阿尔茨海默病供体的细胞比来自年轻供体的细胞扩散更慢，并且这种缺陷可以通过用钙离子载体A23187治疗而部分逆转。这些研究与积累的证据一致，即在细胞水平上，阿尔茨海默病是一种全身性疾病，也是一种大脑疾病。成纤维细胞胞浆钙减少的确切分子基础尚不清楚，但有证据表明它可能具有重要的病理生理学意义。
Aging and Alzheimer disease lead to alterations in calcium homeostasis. The concentration of cytosolic free calcium in cultured skin fibroblasts during aging and Alzheimer disease was determined with the calcium-sensitive fluorescent dyes quin-2 and fura-2. The Alzheimer donors showed a decline of 70% when compared to age-matched controls (P less than 0.001) and 81% when compared to cells from young adult donors (P less than 0.001). This reduction in quin-2-calcium fluorescence does not appear to be due to quenching by heavy metals or alterations in intracellular pH. Similar decreases in free cytosolic calcium were observed with fura-2. In addition, cells from aged and Alzheimer donors spread more slowly than those from young donors, and this deficit can be partially reversed by treatment with the calcium ionophore A23187. These studies agree with accumulating evidence that, at the cellular level, Alzheimer disease is a systemic, as well as cerebral, disease. The precise molecular basis of the decreased cytosolic calcium in fibroblasts is unknown, but there is evidence that it may be pathophysiologically important.