Transcriptome sequencing identifies novel persistent viruses in herbicide resistant wild-grasses.
Transcriptome sequencing identifies novel persistent viruses in herbicide resistant wild-grasses.
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DOI：
10.1038/srep41987
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发表时间：
2017-02-06
影响因子：
4.6
通讯作者：
Edwards R
中科院分区：
文献类型：
--
作者：
Sabbadin F;Glover R;Stafford R;Rozado-Aguirre Z;Boonham N;Adams I;Mumford R;Edwards R
杂草的除草剂抗性在英国很普遍，对多种化学物质的非靶点抗性（NTSR）在杂草控制中特别成问题。作为一个复杂的性状，NTSR是由复杂的进化压力和植物生物群系在植物非生物胁迫耐受性中的作用的日益认识所驱动的，这使得我们对黑草和一年生黑麦草的除草剂抗性和敏感群体的转录组进行测序，以确定内生菌的存在。黑草（Alopecurus myosuroides; AM）人群，显示没有明显的疾病症状，包含三个以前未描述的病毒属于Partitiviridae（AMPV 1和AMPV 2）和弹状病毒科（AMVV 1）的家庭。这些感染在英国黑草种群中广泛存在，并且在一年生黑麦草（Lolium rigidum）、多年生黑麦草（Lolium perenne）和草甸羊茅（Festuca pratensis）中获得了类似病毒的证据。在黑草中，虽然没有建立直接的因果联系，将病毒感染与除草剂抗性联系起来，但转录组测序显示NTSR Peldon人群的感染率很高。这些杂草的广泛感染的特点和持久性的病毒和它们的潜在的进化作用，提高植物耐胁迫机制，包括NTSR值得进一步调查。
Herbicide resistance in wild grasses is widespread in the UK, with non-target site resistance (NTSR) to multiple chemistries being particularly problematic in weed control. As a complex trait, NTSR is driven by complex evolutionary pressures and the growing awareness of the role of the phytobiome in plant abiotic stress tolerance, led us to sequence the transcriptomes of herbicide resistant and susceptible populations of black-grass and annual rye-grass for the presence of endophytes. Black-grass (Alopecurus myosuroides; Am) populations, displaying no overt disease symptoms, contained three previously undescribed viruses belonging to the Partititiviridae (AMPV1 and AMPV2) and Rhabdoviridae (AMVV1) families. These infections were widespread in UK black-grass populations and evidence was obtained for similar viruses being present in annual rye grass (Lolium rigidum), perennial rye-grass (Lolium perenne) and meadow fescue (Festuca pratensis). In black-grass, while no direct causative link was established linking viral infection to herbicide resistance, transcriptome sequencing showed a high incidence of infection in the NTSR Peldon population. The widespread infection of these weeds by little characterised and persistent viruses and their potential evolutionary role in enhancing plant stress tolerance mechanisms including NTSR warrants further investigation.