Anterior Pituitary Progenitor Cells Express Costimulatory Molecule 4Ig-B7-H3
Anterior Pituitary Progenitor Cells Express Costimulatory Molecule 4Ig-B7-H3
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DOI：
10.4049/jimmunol.181.9.6073
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发表时间：
2008-11-01
影响因子：
4.4
通讯作者：
Yamaguchi, Takahiro
中科院分区：
文献类型：
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作者：
Nagai, Yasuhiro;Aso, Hisashi;Yamaguchi, Takahiro
出生后垂体中的干细胞/祖细胞嵌入 Rathke 囊周围的边缘细胞层中。然而，垂体前叶祖细胞的性质和行为仍不清楚。我们从垂体前叶建立了牛垂体前叶祖细胞系（BAPC）-1，该细胞表达干/祖细胞相关基因和几种炎症细胞因子。为了表征和定位这些垂体祖细胞，我们生产了针对 BAPC-1 的 mAb (12B mAb)。 12B mAb 可识别 4Ig-B7-H3 分子，该分子是 T 细胞激活中的共刺激分子和负调节因子。幼年牛 PBMC 中的 WC1(+) γ δ T 细胞表达 4Ig-B7-H3 分子，但成年牛 PBMC 中很少或不表达 4Ig-B7-H3 免疫反应性细胞。牛垂体前叶中的 12B 免疫反应细胞位于 Rathke 育儿袋周围，表达 IL-18 和 MHC II 类。然而，成年牛的 12B 免疫反应细胞数量低于幼牛。 BAPC-1 表达 IL-18 和 MHC 11 类，并表现出吞噬活性。共培养 5 天后，BAPC-1 还具有促进 PBMC 中 CD25 表达的能力，并且阻断 4Ig-B7-H3 X 12B mAb 增强了其 CD25 的表达。此外，在紧邻正中隆起的结节周围以及垂体前叶初级门丛的血管中观察到了免疫反应性细胞1213。这些结果表明，已建立的 BAPC-1 可能源自这些祖细胞，并且具有 4Ig-B7-H3 的祖细胞可能在免疫内分泌网络中发挥关键作用。免疫学杂志，2008，181：6073-6081。
Stem/Progenitor cells in the postnatal pituitary gland are embedded in a marginal cell layer around Rathke's pouch. However, the nature and behavior of anterior pituitary progenitor cells remain unclear. We established bovine anterior pituitary progenitor cell line (BAPC)-1 from the anterior pituitary gland, which expressed stem/progenitor cell-related genes and several inflammatory cytokines. To characterize and localize these pituitary progenitor cells, we produced a mAb (12B mAb) against BAPC-1. The 12B mAb recognized the 4Ig-B7-H3 molecule, which is a costimulatory molecule and negative regulator in T cell activation. WC1(+) gamma delta T cells in young bovine PBMC express the 4Ig-B7-H3 molecule, but few or no 4Ig-B7-H3-immunoreactive cells are expressed in PBMC in adult cattle. The 12B-immunoreactive cells in the bovine anterior pituitary gland were localized around Rathke's pouch and expressed IL-18 and MHC class II. However, the number of 12B-immunoreactive cells was lower in adult than in young cattle. BAPC-1 expressed IL-18 and MHC class 11, and demonstrated phagocytotic activity. BAPC-1 also had the ability to promote CD25 expression in PBMC after 5 days of coculture, and blocking 4Ig-B7-H3 X 12B mAb enhanced their expression of CD25. In addition, the 1213-immunoreactive cells were observed around the pars tuberalis closely bordering the median eminence and in the blood vessels of the primary portal plexus in the anterior pituitary gland. These results suggest that an established BAPC-1 may originate from these progenitor cells, and that the progenitor cells with 4Ig-B7-H3 may play a critical role in the immunoendocrine network. The Journal of Immunology, 2008, 181: 6073-6081.