Membrane fusion of giant unilamellar vesicles of neutral phospholipid membranes induced by La3+
Membrane fusion of giant unilamellar vesicles of neutral phospholipid membranes induced by La3+
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DOI：
10.1021/la049681s
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发表时间：
2004-06-22
期刊：
影响因子：
3.9
通讯作者：
Yamazaki, M
中科院分区：
文献类型：
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作者：
Tanaka, T;Yamazaki, M
生物膜囊泡的膜融合在细胞中发挥着多种重要作用，但其机制尚不清楚且存在争议。在本研究中，我们发现30 muM至1 mM La3+诱导由二油酰磷脂酰胆碱（DOPC）和二棕榈油酰磷脂酰乙醇胺（DPOPE）混合物组成的两个巨大单层囊泡（GUV）的膜融合。我们成功地详细观察了该膜融合的过程。首先，两个 GUV 紧密相连，它们之间有一个由两个双层组成的隔离膜，每个 GUV 各有一个双层。然后，隔离膜边缘的一处突然破裂。这个断裂位点的面积逐渐扩散，直至与GUV完全分离，完成膜融合。在这里，我们提出了一个新的 La3+ 诱导 GUV 膜融合机制的模型（即分区破损模型）。
Membrane fusions of vesicles of biomembranes play various important roles in cells, but their mechanisms are unclear and controversial. In the present study, we found that 30 muM to 1 mM La3+ induced membrane fusion of two giant unilamellar vesicles (GUVs) composed of a mixture of dioleoylphosphatidylcholine (DOPC) and dipalmitoleoylphosphatidylethanolamine (DPOPE). We succeeded in observing a process of this membrane fusion in detail. First, two GUVs became strongly associated, with a partition membrane between them composed of two bilayers, one from each GUV. Then, the partition membrane was suddenly broken at one site on its edge. The area of this breakage site gradually spread, until it was completely separated from the GUV to complete the membrane fusion. Here, we propose a new model (i.e., the partition breakage model) for the mechanism of La3+-induced membrane fusion of GUVs.