The Fission Yeast php2 Mutant Displays a Lengthened Chronological Lifespan
The Fission Yeast php2 Mutant Displays a Lengthened Chronological Lifespan
复制标题
DOI：
10.1271/bbb.130223
复制
发表时间：
2013-07-01
影响因子：
1.6
通讯作者：
Aiba, Hirofumi
中科院分区：
文献类型：
--
作者：
Takuma, Kazuaki;Ohtsuka, Hokuto;Aiba, Hirofumi
粟酒裂殖酵母 php2(+) 基因编码 CCAAT 结合因子复合物的一个亚基。我们发现 php2(+) 基因的破坏延长了裂殖酵母的实际寿命。此外，在热量限制（CR）条件下，Delta php2 突变体的寿命几乎没有延长。 Delta php2 突变体的许多其他表型与 CR 条件下生长的野生型细胞相似，表明 Delta php2 突变体可能经历 CR。该突变体还表现出低呼吸活性，同时伴随着 cyc1(+) 和 rip1(+) 基因表达的降低，这两个基因都参与线粒体电子传递。基于染色质免疫沉淀分析，我们确定 Php2 与粟酒裂殖酵母中 cyc1(+) 和 rip1(+) 上游的 DNA 区域结合。在这里，我们讨论了 Delta php2 突变体的时间寿命延长的可能机制。
The Schizosaccharomyces pombe php2(+) gene encodes a subunit of the CCAAT-binding factor complex. We found that disruption of the php2(+) gene extended the chronological lifespan of the fission yeast. Moreover, the lifespan of the Delta php2 mutant was barely extended under calorie restricted (CR) conditions. Many other phenotypes of the Delta php2 mutant resembled those of wild-type cells grown under CR conditions, suggesting that the Delta php2 mutant might undergo CR. The mutant also showed low respiratory activity concomitant with decreased expression of the cyc1(+) and rip1(+) genes, both of which are involved in mitochondrial electron transport. On the basis of a chromatin immunoprecipitation assay, we determined that Php2 binds to a DNA region upstream of cyc1(+) and rip1(+) in S. pombe. Here we discuss the possible mechanisms by which the chronological lifespan of Delta php2 mutant is extended.