CROSS-FLOW MEMBRANE FILTRATION OF YEAST SUSPENSIONS
CROSS-FLOW MEMBRANE FILTRATION OF YEAST SUSPENSIONS
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DOI：
10.1016/0168-1656(87)90066-6
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发表时间：
1987-02-01
影响因子：
4.1
通讯作者：
CHERYAN, M
中科院分区：
文献类型：
--
作者：
PATEL, PN;MEHAIA, MA;CHERYAN, M
研究了用合成膜收获酵母细胞的可行性。三个错流膜过滤器的通量和细胞产量进行了比较：褶片微过滤器，管式微过滤器和中空纤维超滤模块。所有三个模块的通量随时间呈指数下降。一般而言，在较低的细胞浓度、较低的跨膜压力和较高的流速下观察到较低的结垢速率。在低细胞浓度下，培养基组分在结垢现象中发挥了相对较大的作用。超滤组件的结垢速率远低于微滤器。褶皱板微过滤器经历了快速堵塞、显著的细胞损失和相当大的清洁困难。相反，其他膜组件显示出几乎定量的细胞回收率。的管状微过滤器的通量是超过100 L m-2 h-1（LMH）在酵母细胞浓度为100 g L-1，而中空纤维具有40 LMH在250 g L-1的通量。
The feasibility of harvesting yeast cells using synthetic membranes was studied. Three cross-flow membrane filters were compared in terms of flux and cell yields: a pleated-sheet microfilter, a tubular microfilter and a hollow fiber ultrafiltration module. Flux declined in an exponential manner with time with all three modules. In general, lower fouling rates were observed at lower cell concentrations, lower transmembrane pressures and higher velocity. Media components played a relatively greater role in the fouling phenomenon at low cell concentrations. The ultrafiltration module had much lower fouling rates than the microfilters. The pleated-sheet microfilter experienced rapid plugging, significant cell loss and considerably greater difficulty in cleaning. In contrast, the other membrane modules showed almost quantitative cell recoveries. The flux of the tubular microfilter was more than 100 L m-2 h-1 (LMH) at yeast cell concentrations of 100 g L-1, while the hollow fibers had a flux of 40 LMH at 250 g L-1.