Observation and characterisation of macrophages in zebrafish liver
Observation and characterisation of macrophages in zebrafish liver
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DOI：
10.1016/j.micron.2020.102851
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发表时间：
2020-05-01
期刊：
影响因子：
2.4
通讯作者：
Braet, Filip
中科院分区：
文献类型：
--
作者：
Cheng, Delfine;Morsch, Marco;Braet, Filip
库普弗细胞是肝脏中的巨噬细胞，在哺乳动物和人类中介导免疫相关功能中发挥重要作用。它们以吞噬大量废物复合物、细胞碎片、微生物颗粒甚至恶性细胞的能力而闻名。位置、外观和功能方面是用于识别肝窦这些特征性细胞的重要特征。迄今为止，关于斑马鱼肝脏中巨噬细胞发生的信息有限。因此，我们的目的是通过利用斑马鱼遗传学和多模态相关成像的最新进展来研究斑马鱼模型中肝脏相关巨噬细胞的超微结构和功能方面。在这里，我们报告的发生在斑马鱼肝脏内的巨噬细胞表现出传统的超微结构特征（例如伪足的存在下，广泛的溶酶体器，吞噬溶酶体和弥补类似的3%的肝脏体积）。有趣的是，这些细胞不位于肝组织的窦状血管床内，而是位于肝细胞之间，缺乏吞噬功能。虽然我们的研究结果证明了与其他实验模型的肝巨噬细胞的存在和结构相似性，但它们的功能特征与啮齿动物和人类中描述的枯否细胞明显不同。这些发现表明，与其他动物实验模型相比，斑马鱼肝脏的先天免疫系统具有一些明显不同的特征。这一结论支持了我们对未来研究的呼吁，以便更好地了解居住在斑马鱼肝脏实质细胞之间的巨噬细胞的生理作用。
Kupffer cells are liver-resident macrophages that play an important role in mediating immune-related functions in mammals and humans. They are well-known for their capacity to phagocytose large amounts of waste complexes, cell debris, microbial particles and even malignant cells. Location, appearance and functional aspects are important features used to identify these characteristic cells of the liver sinusoid. To-date, there is limited information on the occurrence of macrophages in zebrafish liver. Therefore, we aimed to characterise the ultrastructural and functional aspects of liver-associated macrophages in the zebrafish model by taking advantage of the latest advances in zebrafish genetics and multimodal correlative imaging. Herein, we report on the occurrence of macrophages within the zebrafish liver exhibiting conventional ultrastructural features (e.g. presence of pseudopodia, extensive lysosomal apparatus, a phagolysosome and making up similar to 3% of the liver volume). Intriguingly, these cells were not located within the sinusoidal vascular bed of hepatic tissue but instead resided between hepatocytes and lacked phagocytic function. While our results demonstrated the presence and structural similarities with liver macrophages from other experimental models, their functional characteristics were distinctly different from Kupffer cells that have been described in rodents and humans. These findings illustrate that the innate immune system of the zebrafish liver has some distinctly different characteristics compared to other animal experimental models. This conclusion underpins our call for future studies in order to have a better understanding of the physiological role of macrophages residing between the parenchymal cells of the zebrafish liver.