Effect of free radical scavengers on endotoxin-induced respiratory muscle dysfunction.
Effect of free radical scavengers on endotoxin-induced respiratory muscle dysfunction.
复制标题
自由基清除剂对内毒素引起的呼吸肌功能障碍的影响。
DOI：
10.1164/ajrccm/148.5.1318
复制
发表时间：
1993
期刊：
影响因子：
--
通讯作者：
DiMarco,A
中科院分区：
文献类型：
--
作者：
Supinski,G;Nethery,D;DiMarco,A
最近的研究表明，自由基有助于脓毒症中观察到的血管功能障碍。然而，之前的工作尚未确定哪种自由基负责产生这些效应，或者在败血症期间肋间肌肉是否受到类似的影响。本研究的目的是使用内毒素介导的脓毒症的仓鼠模型来检查这些问题，其中在处死后从这些动物切除的肌条上评估隔膜和肋间肌功能。研究了几组动物，包括注射（1）盐水、（2）内毒素、（3）内毒素加活性PEG-SOD（超氧化物清除剂）、（4）内毒素加活性PEG-过氧化氢酶（过氧化氢清除剂）、（5）内毒素加DMSO（羟基清除剂）和（6）内毒素加变性PEG-SOD的动物。我们发现，内毒素管理引起膈肌和肋间肌收缩力显着下降。在给予活性自由基清除剂的三组动物中，内毒素的作用减弱。然而，变性PEG-SOD不能保护呼吸肌免受内毒素介导的功能障碍。这些数据表明，膈肌和肋间肌受脓毒症的影响相似;此外，几种自由基物质（超氧离子，过氧化氢和氢氧根离子）在介导这种类型的损伤中发挥作用。
Recent studies have suggested that free radicals contribute to the diaphragmatic dysfunction observed in sepsis. However, previous work has not determined which species of free radicals are responsible for producing these effects or whether the intercostal muscles are affected similarly during sepsis. The purpose of this study was to examine these issues using a hamster model of endotoxin-mediated sepsis in which diaphragm and intercostal muscle function was assessed on muscle strips excised from these animals after killing. Several groups of animals were studied, including animals injected with (1) saline,(2) endotoxin,(3) endotoxin plus active PEG-SOD, a Superoxide scavenger,(4) endotoxin plus active PEG-catalase, a hydrogen peroxide scavenger,(5) endotoxin plus DMSO, a hydroxyl scavenger, and (6) endotoxin plus denatured PEG-SOD. We found that endotoxin administration elicited significant reductions in diaphragm and intercostal muscle contractility. In each of the three groups of animals to which active free radical scavengers were administered, the effects of endotoxin were attenuated. Denatured PEG-SOD did not protect the respiratory muscles from endotoxin-mediated dysfunction, however. These data indicate that both the diaphragm and intercostal muscles are affected similarly by sepsis; moreover, several free radical species (Superoxide ions, hydrogen peroxide, and hydroxyl ions) play a role in mediating this type of injury.