Role of Regulatory B Cells in the Progression of Cervical Cancer
Role of Regulatory B Cells in the Progression of Cervical Cancer
复制标题
调节性 B 细胞在宫颈癌进展中的作用
DOI：
10.1155/2019/6519427
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发表时间：
2019-01-01
影响因子：
4.6
通讯作者：
Ding, Yan
中科院分区：
文献类型：
--
作者：
Chen, Zhifang;Zhu, Yuejie;Ding, Yan
本研究旨在探讨调节性B（布雷格）细胞在宫颈癌中的作用。共纳入70例宫颈癌患者、52例宫颈上皮内瘤变（CIN）患者和40例正常对照。流式细胞仪检测细胞中布雷格细胞的百分率。ELISA法检测血清IL-10水平。分析布雷格细胞与宫颈癌临床特征的相关性。通过体外阻断IL-10检测布雷格细胞对CD 8 + T细胞的抑制作用。宫颈癌组和CIN组CD 19 + CD 5 + CD 1d+布雷格细胞百分率和IL-10水平均显著高于对照组（P < 0.05）。术后布雷格细胞和IL-10水平明显低于术前（P < 0.05）。宫颈癌患者外周血布雷格细胞与IL-10水平呈正相关（r = 0.516）。此外，布雷格细胞百分比与宫颈癌的FIGO分期、淋巴结转移、肿瘤分化程度、HPV感染及肿瘤转移密切相关（P < 0.05）。宫颈癌患者外周血布雷格细胞百分比与CD 8 + T细胞呈负相关（r =-0.669）。经CpG处理后的BMPs培养上清中IL-10水平显著高于非BMPs培养上清（P < 0.05）。与IL-10共培养后，CD 8 + T细胞分泌穿孔素和颗粒酶B的数量显著减少，用特异性抗体阻断IL-10后，这种作用被逆转。布雷格细胞在宫颈癌中升高，并与疾病进展和转移相关。此外，它们可以抑制CD 8 + T细胞的细胞毒性。
This study is to investigate the role of regulatory B (Breg) cells in cervical cancer. In total, 70 cases of cervical cancer, 52 cases of cervical intraepithelial neoplasia (CIN), and 40 normal controls were enrolled. The percentage of Breg cells was detected by flow cytometry. Serum levels of IL-10 were measured by ELISA. The correlation between Breg cells and the clinical characterizations of cervical cancer was analyzed. The inhibition effect of Breg cells on CD8+ T cells was tested by blocking IL-10 in vitro. The percentage of CD19+CD5+CD1d+ Breg cells and the level of IL-10 of patients with cervical cancer or CIN were significantly higher than those in the control group (P < 0.05). And the postoperative levels of Breg cells and IL-10 were significantly lower than the preoperative levels (P < 0.05). Breg cells and the IL-10 level were positively correlated in cervical cancer patients (r = 0.516). In addition, the Breg cell percentage was closely related to the FIGO stages, lymph node metastasis, tumor differentiation, HPV infection, and the tumor metastasis of cervical cancer (P < 0.05). The Breg cell percentage was negatively correlated with CD8+ T cells of cervical cancer patients (r = ‐0.669). The level of IL-10 in the culture supernatant of Bregs treated with CpG was significantly higher than that of non-Bregs (P < 0.05). After coculture with Bregs, the quantity of CD8+ T cells to secrete perforin and Granzyme B was significantly decreased, and this effect was reversed after blocking IL-10 by a specific antibody. Breg cells are elevated in cervical cancer and associated with disease progression and metastasis. Moreover, they can inhibit the cytotoxicity of CD8+ T cells.