Spatial Genetic Structure of the Abundant and Widespread Peatmoss Sphagnum magellanicum Brid.
Spatial Genetic Structure of the Abundant and Widespread Peatmoss Sphagnum magellanicum Brid.
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DOI：
10.1371/journal.pone.0148447
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Stenøien HK
中科院分区：
文献类型：
--
作者：
Kyrkjeeide MO;Hassel K;Flatberg KI;Shaw AJ;Yousefi N;Stenøien HK
产孢子的生物有小的传播单位，使它们能够在各大洲广泛传播。然而，可能存在基因流动和隐蔽物种形成的障碍。常见的单倍体泥炭藓Sphagnummagellanicum分布于北方和南半球，通常被用作泥炭地生态学和泥炭藓生理学研究的模式。尽管它可能在未来几年内成为功能基因组学研究的丰富来源，但令人惊讶的是，人们对该物种的遗传变异性和结构水平知之甚少。在这里，我们首次评估了S.麦哲伦属在其整个分布范围（北方半球和南美洲）上具有空间结构。形态相似的种S.包括阿拉斯加作为比较。总共有195株植物在15个微卫星位点进行了基因分型。从30个样品中获得了两个质体基因座（trnG和trnL）的序列。结果表明，S. alaskense和S.北方太平洋地区的麦哲伦属植物都是二倍体，并且具有相同的基因库。单倍体植物分布于南美洲、欧洲、北美洲东部、北美洲西部和亚洲南部，并存在5个不同分布范围的遗传分化类群。麦哲伦属由几个不同的遗传群组成，它们之间似乎很少或没有基因流动。值得注意的是，二倍体和单倍体的地理分离与在北方半球的其他单倍体泥炭藓属物种中发现的模式惊人地相似。我们的研究结果证实了白令纪和大西洋之间的遗传分工，似乎是泥炭藓类群的一般模式。在形态相似植物的分布范围内，强遗传群体结构的模式需要在未来的功能基因组研究中加以考虑。麦哲伦。
Spore-producing organisms have small dispersal units enabling them to become widespread across continents. However, barriers to gene flow and cryptic speciation may exist. The common, haploid peatmoss Sphagnum magellanicum occurs in both the Northern and Southern hemisphere, and is commonly used as a model in studies of peatland ecology and peatmoss physiology. Even though it will likely act as a rich source in functional genomics studies in years to come, surprisingly little is known about levels of genetic variability and structuring in this species. Here, we assess for the first time how genetic variation in S. magellanicum is spatially structured across its full distribution range (Northern Hemisphere and South America). The morphologically similar species S. alaskense was included for comparison. In total, 195 plants were genotyped at 15 microsatellite loci. Sequences from two plastid loci (trnG and trnL) were obtained from 30 samples. Our results show that S. alaskense and almost all plants of S. magellanicum in the northern Pacific area are diploids and share the same gene pool. Haploid plants occur in South America, Europe, eastern North America, western North America, and southern Asia, and five genetically differentiated groups with different distribution ranges were found. Our results indicate that S. magellanicum consists of several distinct genetic groups, seemingly with little or no gene flow among them. Noteworthy, the geographical separation of diploids and haploids is strikingly similar to patterns found within other haploid Sphagnum species spanning the Northern Hemisphere. Our results confirm a genetic division between the Beringian and the Atlantic that seems to be a general pattern in Sphagnum taxa. The pattern of strong genetic population structuring throughout the distribution range of morphologically similar plants need to be considered in future functional genomic studies of S. magellanicum.