Behavior of host and graft cells in the early remodeling process of rotator cuff defects in a transgenic animal model
Behavior of host and graft cells in the early remodeling process of rotator cuff defects in a transgenic animal model
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DOI：
10.1016/j.jse.2007.07.008
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发表时间：
2008-01-01
影响因子：
3
通讯作者：
Kubo, Toshikazu
中科院分区：
文献类型：
--
作者：
Iwata, Yoshio;Morihara, Toru;Kubo, Toshikazu
自体组织移植是治疗大面积肩袖缺损的方法之一。为了开发适当的策略，以增强骨-肌腱界面和肌腱-肌腱界面处的实体移植物整合，澄清有助于修复和重塑的移植物和宿主细胞的命运是必要的。我们建立了一种新的移植模型，使用绿色荧光蛋白转基因大鼠和野生型大鼠模拟自体移植，以检查宿主和移植细胞在肌腱移植后重建过程中的行为。我们发现，移植后1天，移植物中的宿主细胞开始增殖。宿主细胞从肩峰下滑膜、近端肌腱和骨-肌腱附着处浸润到移植物中。移植物来源的细胞数量随着时间的推移而减少。我们的结果清楚地表明，宿主细胞，而不是移植细胞，是必不可少的肌腱移植后肩袖缺损的重建。
Autologous tissue graft is one of the treatment options for a large rotator cuff defect. To develop appropriate strategies for enhanced solid graft integration at the bone-tendon interface and tendon-tendon interface, clarifying the fate of the graft and host cells that contribute to repair and remodeling is necessary. We have developed a new grafting model using green fluorescent protein-transgenic rats and wild-type rats to simulate autologous transplantation for examining the behavior of the host and graft cells in the remodeling process after tendon grafting. We found that the host cells commenced proliferation in the graft at 1 day after grafting. The host cells infiltrated into the graft from the subacromial synovium, proximal tendon, and bone-tendon insertion. The number of graft-derived cells decreased with time. Our result clearly demonstrated that host cells, rather than graft cells, were essential for rotator cuff remodeling after tendon grafting for rotator cuff defect.