Stimulation of neutrophils by tumor necrosis factor.
Stimulation of neutrophils by tumor necrosis factor.
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DOI：
10.4049/jimmunol.136.11.4220
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发表时间：
1986-06
影响因子：
4.4
通讯作者：
S. Klebanoff;M. Vadas;J. Harlan;L. Sparks;J. Gamble;J. Agosti;A. Waltersdorph
中科院分区：
文献类型：
--
作者：
S. Klebanoff;M. Vadas;J. Harlan;L. Sparks;J. Gamble;J. Agosti;A. Waltersdorph
人重组肿瘤坏死因子（TNF）对中性粒细胞呼吸爆发和脱粒有微弱的直接刺激作用。的刺激，测量碘化，H2 O2的生产，和溶菌酶的释放，大大增加了unopsonized酵母多糖在反应混合物中的存在下，这是与增加摄入的酵母多糖的效果。TNF不作为调理素，而是与中性粒细胞反应以增加其吞噬活性。通过碘化间接测量的TNF依赖性吞噬作用被单克隆抗体（Mab）60.1和60.3抑制，所述单克隆抗体（Mab）60.1和60.3识别中性粒细胞的C3 bi受体/粘附促进表面糖蛋白上的不同表位。其他中性粒细胞刺激剂，即N-甲酰基-甲硫氨酰-亮氨酰-苯丙氨酸，钙离子载体A23187，和佛波醇肉豆蔻乙酸酯，也增加碘化酵母聚糖的存在下，与TNF，这些刺激剂的效果被抑制单克隆抗体60.1和60.3，而相反，TNF，它们的刺激碘化是不受单克隆抗体针对TNF。TNF可能是中性粒细胞的天然刺激物，其促进对内皮细胞和颗粒的粘附，导致吞噬作用增加、呼吸爆发活性和脱粒。
Human recombinant tumor necrosis factor (TNF) was shown to be a weak direct stimulus of the neutrophil respiratory burst and degranulation. The stimulation, as measured by iodination, H2O2 production, and lysozyme release, was considerably increased by the presence of unopsonized zymosan in the reaction mixture, an effect which was associated with the increased ingestion of the zymosan. TNF does not act as an opsonin but, rather, reacts with the neutrophil to increase its phagocytic activity. TNF-dependent phagocytosis, as measured indirectly by iodination, is inhibited by monoclonal antibodies (Mab) 60.1 and 60.3, which recognize different epitopes on the C3bi receptor/adherence-promoting surface glycoprotein of neutrophils. Other neutrophil stimulants, namely N-formyl-methionyl-leucyl-phenylalanine, the Ca2+ ionophore A23187, and phorbol myristic acetate, also increase iodination in the presence of zymosan; as with TNF, the effect of these stimulants is inhibited by Mab 60.1 and 60.3, whereas, in contrast to that of TNF, their stimulation of iodination is unaffected by an Mab directed against TNF. TNF may be a natural stimulant of neutrophils which promotes adherence to endothelial cells and to particles, leading to increased phagocytosis, respiratory burst activity, and degranulation.