Cysteine-rich protein-templated silver nanoclusters as a fluorometric probe for mercury(ii) detection
Cysteine-rich protein-templated silver nanoclusters as a fluorometric probe for mercury(ii) detection
复制标题
富含半胱氨酸的蛋白质模板银纳米团簇作为汞（ii）检测的荧光探针
DOI：
10.1039/c8ay02662a
复制
发表时间：
2019
影响因子：
3.1
通讯作者：
Yang Jingxia
中科院分区：
文献类型：
--
作者：
Zhou Yongquan;Wang Jinjie;Yang Guang;Ma Shuyi;Zhang Min;Yang Jingxia
以角蛋白为模板，NaBH4为还原剂，探索了一种在室温下1h内制备出稳定的银纳米团簇的简便方法。角蛋白是一类富含半胱氨酸的结构蛋白，广泛存在于羊毛、毛发和羽毛中。所制备的AgNCs具有较高的荧光强度和在705 nm处的稳定发射。在4℃的黑暗中放置3个月后，其荧光强度下降了20%，并用于检测Hg2+离子。检测下限低(6.5 nm)，线性范围为13.7~10μM。此外，利用UV-Vis、X-射线衍射仪、透射电子显微镜和X射线光电子能谱等分析技术研究了AgNC与Hg2+离子的作用机理。
In this paper, we explored a convenient method for preparing stable silver nanoclusters (AgNCs) within 1 h at room temperature using keratin as a template and NaBH4 as a reducing agent. As a group of cysteine-rich structural proteins, keratins are abundant in wool, hair and feathers. The prepared AgNCs exhibited high fluorescence intensity and stable emission at 705 nm. Their fluorescence intensity decreased by <20% after 3 months of storage in the dark at 4 °C. The AgNCs were then used to detect Hg2+ ions with high selectivity. The detection limit can be low (6.5 nM), and the linear range was from 13.7 nM to 10 μM. Furthermore, the mechanism of the interaction of AgNCs with Hg2+ ions was studied using various analytical techniques, such as UV-vis, XRD, TEM and XPS.