Cladosporium Avr2 inhibits tomato Rcr3 protease required for Cf-2-dependent disease resistance
Cladosporium Avr2 inhibits tomato Rcr3 protease required for Cf-2-dependent disease resistance
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DOI：
10.1126/science.1111404
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发表时间：
2005-06-17
期刊：
影响因子：
56.9
通讯作者：
de Wit, PJGM
中科院分区：
文献类型：
--
作者：
Rooney, HCE;van 't Klooster, JW;de Wit, PJGM
植物如何识别病原体并激活防御仍然是个谜。病原体无毒（Avr）蛋白和植物抗病蛋白之间的直接相互作用是例外而不是规则。在感染过程中，黄枝孢菌将 Avr2 蛋白分泌到番茄叶的质外体中，并且在细胞外富含亮氨酸的重复受体样 Cf-2 蛋白存在的情况下，触发过敏反应 (HR)，该反应也需要细胞外番茄半胱氨酸蛋白酶 Rcr3。我们在此表明 Avr2 结合并抑制 Rcr3，并提出 Rcr3-Avr2 复合物使 Cf-2 蛋白能够激活 HR。
How plants recognize pathogens and activate defense is still mysterious. Direct interaction between pathogen avirulence (Avr) proteins and plant disease resistance proteins is the exception rather than the rule. During infection, Cladosporium fulvum secretes Avr2 protein into the apoplast of tomato leaves and, in the presence of the extracellular leucine-rich repeat receptor-like Cf-2 protein, triggers a hypersensitive response (HR) that also requires the extracellular tomato cysteine protease Rcr3. We show here that Avr2 binds and inhibits Rcr3 and propose that the Rcr3-Avr2 complex enables the Cf-2 protein to activate an HR.