Anti-nociceptive, anti-inflammatory and anti-arthritic activities of pregnane glycosides from the root bark of Periploca sepium Bunge
Anti-nociceptive, anti-inflammatory and anti-arthritic activities of pregnane glycosides from the root bark of Periploca sepium Bunge
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Periploca sepium Bunge 根皮孕烷苷的抗伤害、抗炎和抗关节炎活性
DOI：
10.1016/j.jep.2020.113345
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发表时间：
2021
影响因子：
5.4
通讯作者：
Zuo Jian-Ping
中科院分区：
文献类型：
--
作者：
Lin Ze-Min;Liu Yu-Ting;Huang Yue-Teng;Yang Xiao-Qian;Zhu Feng-Hua;Tang Wei;Zhao Wei-Min;He Shi-Jun;Zuo Jian-Ping
民族药理学相关性杠柳（Periploca sepium Bunge）（P. sepium）在传统中药（TCM）中用于治疗自身免疫性疾病，特别是类风湿性关节炎。杠柳（Periploca sepium periplosides，PePs）是从杠柳（Periploca sepium）的根皮中分离得到的一种新植物。Sepium，其特征在于作为心脏糖苷-游离的姜烷糖苷级分，预期具有对炎性关节炎的治疗潜力。研究的目的本研究旨在评价PePs的抗伤害性、抗炎性和抗关节炎活性作用。采用2，4-二硝基氟苯（DNFB）致小鼠耳肿胀模型和角叉菜胶致小鼠足肿胀模型观察PePs的抗炎活性。通过评价大鼠佐剂诱导的关节炎（AIA）和小鼠胶原诱导的关节炎（CIA）中的关节炎症和关节炎病理来研究PePs的抗关节炎活性。进一步应用植物血凝素M（PHA-M）诱导的人外周血单个核细胞（PBMCs）来评估PePs对IFN-γ和IL-17产生的抑制活性。此外，口服给药PeP通过减少DNFB诱导的小鼠耳水肿和角叉菜胶诱导的大鼠爪水肿而表现出抗炎活性。此外，口服PeP治疗可改善啮齿类动物关节炎的关节肿胀并减轻骨侵蚀，治疗益处部分归因于抑制促炎细胞因子如IFN-γ和IL-17。此外，PePs抑制增殖以及IFN-γ和IL-17的分泌在PHA-M引起的人PBMCsin a concentration dependentmannes. ConclusionsTogether，我们的研究结果证明了传统的使用杠柳用于治疗与炎症和疼痛相关的疾病。
Ethnopharmacological relevancePeriploca sepiumBunge (P. sepium) is used in traditional Chinese medicine (TCM) for the treatment of autoimmune diseases, particularly rheumatoid arthritis. Periploca sepium periplosides (PePs), isolated from the root bark ofP. sepium, characterized as the cardiac glycosides-free pregnane glycosides fraction, is expected to possess therapeutic potential on inflammatory arthritis.Aim of the studyThe current study is designed to evaluate the anti-nociceptive, anti-inflammatory and anti-arthritic activities effects of the PePs.Materials and methodsThe anti-nociceptive activity of PePs was examined in the writhing test and hot-plate test in mice. The anti-inflammatory activity of PePs was determined by the 2, 4-dinitro-1-fluorobenzene (DNFB)-induced ear edema model and the carrageenan induced paw edema model in mice. The anti-arthritic activity of PePs was investigated by evaluating the joint inflammation and arthritis pathology in rat adjuvant induced arthritis (AIA) and murine collagen induced arthritis (CIA). Phytohaemagglutinin M (PHA-M) -elicited human peripheral blood mononuclear cells (PBMCs) were further applied to assess the suppressive activity of PePs on IFN-γ and IL-17 production.ResultsPePs treatment markedly decreased the acetic acid-induced visceral nociceptive response and increased the hot-plate pain threshold. Further, oral administration of PePs exhibited anti-inflammatory activity by decreasing DNFB-induced ear edema in mice and carrageenan-induced paw edema in rats. Moreover, oral treatment of PePs ameliorated joint swelling and attenuated bone erosion in rodent arthritis, and the therapeutic benefits were partially attributed to the suppression of proinflammatory cytokines such IFN-γ and IL-17. Moreover, PePs suppressed the proliferation as well as IFN-γ and IL-17 secretion in PHA-M-elicited human PBMCs in a concentration dependent manner.ConclusionsTaken together, our results justified the traditional use of Periploca sepium Bunge for the treatment of diseases associated with inflammation and pain.