Quantitative Orientation-Independent Differential Interference Contrast (DIC) Microscopy Coupled with Orientation-Independent Polarization Microscopy.
Quantitative Orientation-Independent Differential Interference Contrast (DIC) Microscopy Coupled with Orientation-Independent Polarization Microscopy.
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定量与方向无关的微分干涉 (DIC) 显微镜与与方向无关的偏振显微镜相结合。
DOI：
10.1017/s1431927607075186
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Inoué,Shinya
中科院分区：
文献类型：
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作者：
Shribak,Michael;Lafountain,James;Biggs,David;Inoué,Shinya
微分干涉对比 (DIC) 显微镜广泛用于观察未染色、透明的活细胞和分离的细胞器的结构和运动，产生光学相位梯度的单色阴影图像。偏光显微镜 (Pol) 揭示了由于形式双折射、固有双折射、应力双折射等而产生的结构各向异性。DIC 和 Pol 相互补充，例如，在活的分裂细胞中，DIC 图像将清楚地显示染色体，而 Pol 图像将描绘纺锤体中双折射微管的分布。然而，这两种方法都有相同的缺点：它们需要样本相对于光学系统的正确方向才能获得最佳结果。我们在这里描述方向无关的微分干涉对比（OI-DIC）显微镜，它记录显微样本内的相位梯度，与其方向无关[1]。 OI-DIC 生成代表样本中光学相位分布的图像，即高保真度干涉显微镜图像。与其他形式的干涉显微镜不同，OI-DIC 不需要狭窄的照明锥。这个新系统可能是为数不多的（即使不是唯一的）显微镜系统之一，它可以在最高的数值孔径下生成干涉显微镜图像。
Differential interference contrast (DIC) microscopy is widely used to observe structure and motion in unstained, transparent living cells and isolated organelles, producing a monochromatic shadowcast image of optical phase gradient. Polarized light microscopy (Pol) reveals structural anisotropy due to form birefringence, intrinsic birefringence, stress birefringence, etc. DIC and Pol complement each other as, for example, in a live dividing cell, the DIC image will clearly show the chromosomes while the Pol image will depict the distribution of the birefringent microtubules in the spindle. Both methods, however, have the same shortcomings: they require the proper orientation of a specimen in relation to the optical system in order to achieve best results.We describe here orientation-independent differential interference contrast (OI-DIC) microscopy, which records phase gradients within microscopic specimens independently of their orientation [1]. OI-DIC generates images representing the distribution of optical phase in the specimen, ie, high fidelity interference microscope images. Unlike in other forms of interference microscopes, OI-DIC does not require a narrow illuminating cone. This new system is probably one of the few, if not the only, microscope system, which allows the generation of interference microscope images at highest NAs.