Skin penetration of topically applied white mustard extract and its effects on epidermal Langerhans cells and cytokines
Skin penetration of topically applied white mustard extract and its effects on epidermal Langerhans cells and cytokines
复制标题
外用白芥末提取物的皮肤渗透性及其对表皮朗格汉斯细胞和细胞因子的影响
DOI：
10.1016/j.ijpharm.2013.09.015
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发表时间：
2013-11-30
影响因子：
5.8
通讯作者：
Xu, Yuehong
中科院分区：
文献类型：
--
作者：
Guo, Xiucai;Lu, Haoyang;Xu, Yuehong
白芥（Sinapis alba L.）是一种传统中药，在我国临床广泛用于夏季经皮给药预防和治疗冬季常见病哮喘、支气管炎等。本研究旨在研究白芥末提取物的皮肤渗透行为，以阐明其免疫调节活性的可能机制。提取物的主要活性化合物硫氰酸芥子碱 (ST) 被用作标记物。在体外和体内检查了白芥末提取物中 ST 的皮肤渗透性。使用 Franz 扩散池对离体豚鼠无毛皮肤进行的体外研究表明，ST 可以渗透皮肤并在皮肤中积累。对豚鼠无毛皮肤进行24 h体内研究，然后切取皮肤进行冰冻切片，提取切片中的ST以定量不同皮层的药物量。 ST的详细分布表明它在表皮中积累，尤其是在角质层中。用白芥末提取物处理24小时后，用ATP酶对皮肤进行染色，并通过图像分析系统将表皮LC的形态参数与未处理的对照进行比较。观察到 LC 密度统计显着降低，形状因子增加。在不同时间点用白芥末提取物处理后，还测量了与 LC 迁移相关的细胞因子，包括白细胞介素 1 β (IL-1 β) 和肿瘤坏死因子 α (TNF-α)。与未处理组相比，白芥末提取物显着增强了 IL-1 β 和 TNF α 的释放。局部白芥末治疗后，LC 的形态变化和局部细胞因子的释放可以解释白芥末提取物对抗哮喘和支气管炎的活性。 c 2013 Elsevier B.V. 保留所有权利。
White mustard (Sinapis alba L.), a traditional Chinese medicine, is widely used in China for clinical prevention and treatment of the common winter diseases of asthma and bronchitis by percutaneous administration in the summer. The present study is to investigate the skin penetration behavior of white mustard extract to elucidate the possible mechanism underlying its immune regulation activity. The principle active compound of the extract, sinapine thiocyanate (ST), was used as a marker. The skin penetration of ST in white mustard extract was examined in vitro and in vivo. In vitro study on excised guinea pig hairless skin using Franz diffusion cell revealed ST can permeate through the skin and also accumulate in the skin. In vivo study was carried out on the guinea pig hairless skin for 24 h, and then skin was excised for frozen section, ST from the sections were extracted to quantify the amount of drug in different skin layers. The detailed distribution of ST showed that it accumulated in the epidermis, especially in the stratum corneum. After treatment with white mustard extract for 24 h, the skin was stained with ATPase, and the morphometric parameters of epidermal LCs were compared to the untreated control through image-analysis system. A statistically significant reduction in LC density and increase in shape factor were observed. Cytokines related to LCs migration including interleukin 1 beta (IL-1 beta) and tumor necrosis factor alpha (TNF-alpha) were also measured after white mustard extract treated at different time points. Compared to the untreated group, white mustard extract significantly enhanced the release of IL-1 beta and TNF alpha. The morphometric changes of LCs and the local cytokine release after topical white mustard treatment may explain the activity of the white mustard extract against asthma and bronchitis. c 2013 Elsevier B. V. All rights reserved.