Detection and identification of Vibrio scophthalmi in the intestinal microbiota of fish and evaluation of host specificity
Detection and identification of Vibrio scophthalmi in the intestinal microbiota of fish and evaluation of host specificity
复制标题
鱼类肠道菌群中斯科氏弧菌的检测、鉴定及宿主特异性评价
DOI：
10.1046/j.1365-2672.2002.01697.x
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发表时间：
2002
影响因子：
4
通讯作者：
A. Blanch
中科院分区：
文献类型：
--
作者：
M. Cerdà;A. Blanch
目的：目的研制一种检测鱼肠道中弧菌的种特异性探针（VSV 3），并应用该探针研究鱼肠道中弧菌的宿主特异性。
方法和结果：根据16 SrRNA基因（rDNA）可变区V3设计了一种特异性探针（VSV 3）。用DNA-DNA杂交法和菌落杂交法检测其特异性。用DNA-DNA杂交法和菌落杂交法检测探针的灵敏度。该方法的检出限为150 pg或10 cfu。 当以1：10和1：100的比例存在时，在由混合培养物构成的实验样品中检测到眼球弧菌细胞。 VSV 3探针也被证明是可靠的检测鱼肠道样品中的猫眼弧菌。
结论：VSV 3探针可用于16 S rDNA扩增的菌落杂交或DNA-DNA杂交检测。初步研究结果表明，大眼弧菌对大菱鲆可能具有一定的宿主特异性。
研究的意义和影响：VSV 3探针为生态学研究提供了有用的工具。
Aims: To develop a species‐specific probe (VSV3) for the detection of Vibrio scophthalmi in fish intestine and to apply this probe to study the host specificity of V. scophthalmi.
Methods and Results: A specific probe (VSV3) based on the variable region V3 of the 16S rRNA gene (rDNA) was designed. Its specificity was tested by DNA–DNA hybridization and by colony hybridization. No cross‐hybridization was found. The sensitivity of the probe was tested both by DNA–DNA hybridization and by colony hybridization. The detection limit of V. scophthalmi 16S rDNA was 150 pg or 10 cfu. Vibrio scophthalmi cells were detected in experimental samples constituted by mixed cultures when present in proportions of 1 : 10 and 1 : 100. The VSV3 probe also proved to be reliable for the detection of V. scophthalmi in samples of fish intestine.
Conclusions: The VSV3 probe can be used for the detection of V. scophthalmi in colony hybridization or DNA–DNA hybridization of amplified 16S rDNA. Preliminary results indicate that V. scophthalmi may present certain host specificity for turbot.
Significance and Impact of the Study: The VSV3 probe provides a useful tool for ecological studies.
DOI：
10.1073/pnas.82.20.6955
发表时间：
1985-01-01
影响因子：
11.1
作者：
LANE, DJ;PACE, B;PACE, NR
通讯作者：
PACE, NR