Role of glycoprotein Ibα in phagocytosis of platelets by macrophages
Role of glycoprotein Ibα in phagocytosis of platelets by macrophages
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DOI：
10.1111/j.1537-2995.2006.01034.x
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发表时间：
2006-12-01
期刊：
影响因子：
2.9
通讯作者：
Akkerman, Jan-Willem N.
中科院分区：
文献类型：
--
作者：
Badlou, Bahram A.;Spierenburg, Gerrit;Akkerman, Jan-Willem N.
背景：0 ~ 4℃的血小板（PLT）储存可抑制细菌繁殖，但会诱导糖蛋白（GP） Ib α聚集，引发巨噬细胞吞噬，降低输血后细菌的存活率。寻找一种方法来检测冷诱导的GPIb α参与吞噬作用的变化。研究设计和方法：分离人plt，并在0℃下保存48小时。GPIb α上针对氨基酸（AA） 1-35的植红蛋白（PE）标记抗体（AN51-PE）的结合与成熟单核THP-1细胞对plt的吞噬进行比较，通过荧光活化细胞分选分析。结果：新分离的plt检测到an51 - pe阳性颗粒，其吞噬率低于5%。低温贮藏导致AN51-PE结合减少，吞噬能力增加。已知干扰巨噬细胞识别GPIb α簇的n -乙酰氨基葡萄糖恢复了AN51-PE与冷藏plt的正常结合并抑制了吞噬作用。结论：抗AA 1-35抗体与GPIb α结合反映了GPIb α的变化，使plt成为巨噬细胞吞噬的靶标。
BACKGROUND: Platelet (PLT) storage at 0 to 4 degrees C suppresses bacterial multiplication, but induces clusters of glycoprotein (GP) Ib alpha that trigger their phagocytosis by macrophages and reduce their survival after transfusion. A method was sought that detects cold-induced changes in GPIb alpha involved in phagocytosis.STUDY DESIGN AND METHODS: Human PLTs were isolated and stored for up to 48 hours at 0 degrees C. Binding of a phycoerythrin (PE)-labeled antibody directed against amino acids (AA) 1-35 on GPIb alpha (AN51-PE) was compared with phagocytosis of PLTs by matured monocytic THP-1 cells, analyzed by fluorescence-activated cell sorting.RESULTS: Freshly isolated PLTs were detected as a single population of AN51-PE-positive particles and showed less than 5 percent phagocytosis. Cold storage led to a decrease in AN51-PE binding and an increase in phagocytosis. N-Acetylglucosamine, known to interfere with macrophage recognition of GPIb alpha clusters, restored normal AN51-PE binding to cold-stored PLTs and suppressed phagocytosis.CONCLUSIONS: It is concluded that binding of an antibody against AA 1-35 on GPIb alpha reflects changes in GPIb alpha that make PLTs targets for phagocytosis by macrophages.