Offsetting effects of Wolbachia infection and heat shock on sperm production in Drosophila simulans: analyses of fecundity, fertility and accessory gland proteins.
Offsetting effects of Wolbachia infection and heat shock on sperm production in Drosophila simulans: analyses of fecundity, fertility and accessory gland proteins.
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沃尔巴克氏体感染和热休克对模拟果蝇精子产生的抵消作用：生育力、生育力和辅助腺蛋白的分析。
DOI：
10.1093/genetics/155.1.167
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发表时间：
2000
期刊：
影响因子：
3.3
通讯作者：
Karr,TL
中科院分区：
文献类型：
--
作者：
Snook,RR;Cleland,SY;Wolfner,MF;Karr,TL
在模拟果蝇中感染细胞内共生体Wolbachia pipientis，由于不能正确启动二倍体发育（细胞质不相容性，CI）而导致卵死亡。Wolbachia感染和生殖因素之间的关系影响男性健身还没有得到很好的研究。在这里，我们比较感染和未感染的菌株D。（2）雄性不育;（3）两种附属腺蛋白Acp 26 Aa或Acp 36 De的转移和加工。在成年后的前10天内，感染的雄性比未感染的雄性产生的精子囊显著减少，并且在不同的交配条件下，感染的雄性的生育能力比未感染的雄性低。这种生育力的影响是由于既不受感染和未受感染的男性之间的差异，在转移和随后的处理的附属腺蛋白的女性，也不存在沃尔巴克氏体在成熟的精子。我们发现，热休克，这是已知的CI表达减少，增加精子生产在更大程度上感染相比，未感染的男性，这表明精子生产和热休克之间可能存在联系。考虑到这些结果，沃尔巴克氏体和热休克在介导雄配子产生中的作用可能是了解自然种群感染动态的重要参数。
Infection inDrosophila simulanswith the endocellular symbiontWolbachia pipientisresults in egg lethality caused by failure to properly initiate diploid development (cytoplasmic incompatibility, CI). The relationship between Wolbachia infection and reproductive factors influencing male fitness has not been well examined. Here we compare infected and uninfected strains ofD. simulansfor (1) sperm production, (2) male fertility, and (3) the transfer and processing of two accessory gland proteins, Acp26Aa or Acp36De. Infected males produced significantly fewer sperm cysts than uninfected males over the first 10 days of adult life, and infected males, under varied mating conditions, had lower fertility compared to uninfected males. This fertility effect was due to neither differences between infected and uninfected males in the transfer and subsequent processing of accessory gland proteins by females nor to the presence of Wolbachia in mature sperm. We found that heat shock, which is known to decrease CI expression, increases sperm production to a greater extent in infected compared to uninfected males, suggesting a possible link between sperm production and heat shock. Given these results, the roles Wolbachia and heat shock play in mediating male gamete production may be important parameters for understanding the dynamics of infection in natural populations.