Identification and cell type specificity of the tyrosine hydroxylase gene promoter.
Identification and cell type specificity of the tyrosine hydroxylase gene promoter.
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酪氨酸羟化酶基因启动子的鉴定和细胞类型特异性。
DOI：
10.1093/nar/15.5.2363
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发表时间：
1987
影响因子：
14.9
通讯作者：
Chikaraishi,DM
中科院分区：
文献类型：
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作者：
Harrington,CA;Lewis,EJ;Krzemien,D;Chikaraishi,DM
用大鼠酪氨酸羟化酶（TH）cDNA克隆的切口翻译片段，从λ噬菌体文库中分离出编码TH基因的基因组DNA。我们确定了TH RJJA合成的起始位点，并对初级转录本和5′侧翼区的1100个碱基进行了测序。在表达TH的几种大鼠组织和大鼠嗜铬细胞（PC）中，转录本的5′端是相同的。从用地塞米松刺激的PC细胞制备的RNA也映射到相同的转录起始位点。起始位点上游的序列含有典型的TATA盒，但没有明显的CAAT盒。当TH基因5′侧翼区的一部分（−773至+27）与氯霉素乙酰转移酶（CAT）基因融合时，它促进了CAT在嗜铬细胞瘤细胞和GH细胞中的表达，但在两种神经肿瘤细胞系RT 4-D和B103中不起作用，在几种非神经细胞系中也不起作用。这表明TH基因的该区域具有赋予TH启动子组织限制性表达的特征。
Genomic DNA encoding the rat tyrosine hydroxylase (TH) gene was isolated from a lambda phage library using a nick-translated fragment froa a cDNA clone for rat TH. We have determined the Initiation site for TH RJJA synthesis and have sequenced 1100 bases of the primary transcript and 5′ flanking region. The 5′ end of the transcript is the same in several rat tissues in which TH is expressed as well as in rat pheochromocytooa cells (PC). RNA prepared from PC cells that had been stimulated with dexamethasone also mapped to the same transcription start site. Sequence upstream from the initiation site contains the canonical TATA box, but no apparent CAAT box. When a portion of the 5′ flanking region of the TH gene (−773 to + 27) is fused to the chloramphenicol acetyltransferase (CAT) gene, it promotes expression of CAT in pheochromocytoma cells and GH, cells, but not in two neural tumour lines, RT4-D and B103, nor in several non neural cell lines. This suggests that this region of the TH gene has features that confer tissue-restricted expression on the TH promoter.
大鼠肾上腺髓质和脑组织酪氨酸羟化酶 mRNA 水平的检测和调节。
DOI：
--
发表时间：
1983
期刊：
Cold Spring Harbor Symposia on Quantitative Biology
影响因子：
--
作者：
J. Mallet;N. Faucon Biguet;M. Buda;A. Lamouroux;D. Samolyk
通讯作者：
D. Samolyk