A preliminary genetic linkage map of chrysanthemum (Chrysanthemum morifolium) cultivars using RAPD, ISSR and AFLP markers
A preliminary genetic linkage map of chrysanthemum (Chrysanthemum morifolium) cultivars using RAPD, ISSR and AFLP markers
复制标题
利用 RAPD、ISSR 和 AFLP 标记绘制菊花 (Chrysanthemum morifolium) 品种的初步遗传连锁图谱
DOI：
10.1016/j.scienta.2010.03.028
复制
发表时间：
2010-06-28
影响因子：
4.3
通讯作者：
Li, Fengtong
中科院分区：
文献类型：
--
作者：
Zhang, Fei;Chen, Sumei;Li, Fengtong
采用RAPD、ISSR和AFLP标记相结合的双重假测交作图策略，对142个高亲杂交组合“玉华落樱”ד傲云寒消”的F1代进行基因型分析，构建了菊花遗传连锁图谱。共使用了567个多态性标记，其中RAPD标记153个，ISSR标记61个，AFLP标记353个。567个标记中有336个（60%）在两个亲本图谱上聚在一起，剩下231个（40%）标记不连锁。在‘玉华落樱’连锁图上，共标记210个，其中测交标记116个，互交标记94个，分布在12个主连锁群和32个次连锁群（8个三联体和24个双联体）中，覆盖范围为1034cM，相邻标记间的平均图距为6.2cM。在“傲云寒消”连锁图中，由113个测交标记和77个互交标记组成的190个标记被划分为9个主连锁群和24个次连锁群，基因组覆盖率为1095cM，相邻标记间的平均距离为6.9cM。根据两个亲本图谱共有的64个互交标记，建立了6对同源连锁群。该图谱为进一步定位菊花的数量性状基因座（QTL）和分子标记辅助育种奠定了基础。(C)2010爱思唯尔有限公司版权所有。
A genetic linkage map of chrysanthemum (Chrysanthemum morifolium) was constructed by genotyping 142 F-1 progeny of the hi-parental cross 'Yuhualuoying' x 'Aoyunhanxiao' with a combination of RAPD, ISSR and AFLP markers in a double pseudo-testcross mapping strategy. A total of 567 polymorphic markers, including 153 RAPDs, 61 ISSRs and 353 AFLPs, were used in linkage mapping. 336 of 567 (60%) markers were grouped on the two parental maps, leaving 231 (40%) markers unlinked. In the 'Yuhualuoying' linkage map, 210 markers including 116 testcross and 94 intercross markers were placed in 12 major and 32 minor (8 triplets and 24 doublets) linkage groups, covering 1034 cM with an average map distance of 6.2 cM between adjacent markers. In 'Aoyunhanxiao' linkage map, 190 markers consisting of 113 testcross and 77 intercross markers were resolved into 9 major and 24 minor linkage groups, with genome coverage of 1095 cM and a mean inter-marker separation of 6.9 cM between adjacent markers. Six pairs of homologous linkage groups were established on the basis of 64 intercross markers shared by the two parental maps. The maps lay a foundation for further quantitative traits loci (QTL) mapping and marker-assisted breeding of chrysanthemum. (C) 2010 Elsevier B.V. All rights reserved.