Magnetic Glyconanoparticles as a Versatile Platform for Selective Immunolabeling and Imaging of Cells
Magnetic Glyconanoparticles as a Versatile Platform for Selective Immunolabeling and Imaging of Cells
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DOI：
10.1021/bc1003923
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发表时间：
2011-02-01
影响因子：
4.7
通讯作者：
Penades, Soledad
中科院分区：
文献类型：
--
作者：
Garcia, Isabel;Gallo, Juan;Penades, Soledad
描述了一种基于磁性糖纳米粒(糖铁酸盐)的多功能纳米平台，以附着定向良好的抗体。采用一种高效的配体交换工艺制备了含两亲性碳水化合物和脂肪族乙二醇链的6 nm大小的水溶性核壳Fe3O4@Au纳米粒子。通过Fc受体(蛋白G)的羧基共价固定化使得免疫球蛋白G能够成功地定向捕获到磁性葡萄糖纳米颗粒上。通过不同的技术对构建物的结构和生物功能进行彻底的表征。通过磁共振成像(MRI)和荧光技术证明了抗体-磁性糖纳米粒结合物对细胞的选择性免疫标记。
A versatile nanoplatform based on magnetic glyconanoparticles (glyco-ferrites) to attach well-oriented antibodies is described. An efficient ligand exchange process has been used to prepare water-soluble 6-nm-sized core shell Fe3O4@Au nanoparticles bearing amphiphilic carbohydrates and aliphatic ethylene glycol chains ended in a carboxyl group. The covalent immobilization through the carboxyl group of an Fc receptor (protein G) enables successful well-oriented capture of immunoglobulins G onto the magnetic glyconanoparticle. A thorough characterization of structure and biofunctionality of the constructs is carried out by different techniques. The selective immunolabeling of cells by the antibody-magnetic glyconanoparticle conjugates is demonstrated by magnetic resonance imaging (MRI), as well as by fluorescence techniques.