A fluorescent toolkit for spatiotemporal tracking of apoptotic cells in living Drosophila tissues
A fluorescent toolkit for spatiotemporal tracking of apoptotic cells in living Drosophila tissues
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DOI：
10.1242/dev.149807
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发表时间：
2017-10-15
期刊：
影响因子：
4.6
通讯作者：
Suzanne, Magali
中科院分区：
文献类型：
--
作者：
Schott, Sonia;Ambrosini, Arnaud;Suzanne, Magali
凋亡细胞非但不是被动的，还会影响它们所处的环境。例如，它们促进组织折叠、成肌细胞融合和调节肿瘤生长。了解凋亡细胞的作用需要它们在活组织中进行有效的跟踪，这是一项目前具有挑战性的任务。为了在发育中的果蝇组织中容易地发现凋亡细胞，我们建立了一系列表达caspase活性的不同荧光感受器的苍蝇系。我们发现其中三个报告分子(GFP、Cerulean和Venus衍生的分子)在凋亡细胞和程序性细胞死亡的整个过程中都被特异性地检测到。这些记者可以直接在活组织内对凋亡细胞进行特定的可视化，而不需要任何采集后处理。它们克服了迄今为止开发的其他细胞凋亡检测方法的局限性，值得注意的是，它们可以与任何类型的荧光团结合。
Far from being passive, apoptotic cells influence their environment. For example, they promote tissue folding, myoblast fusion and modulate tumor growth. Understanding the role of apoptotic cells necessitates their efficient tracking within living tissues, a task that is currently challenging. In order to easily spot apoptotic cells in developing Drosophila tissues, we generated a series of fly lines expressing different fluorescent sensors of caspase activity. We show that three of these reporters (GFP-, Cerulean- and Venus-derived molecules) are detected specifically in apoptotic cells and throughout the whole process of programmed cell death. These reporters allow the specific visualization of apoptotic cells directly within living tissues, without any post-acquisition processing. They overcome the limitations of other apoptosis detection methods developed so far and, notably, they can be combined with any kind of fluorophore.