Defining the growth conditions and promoter-proximal DNA sequences required for activation of gene expression by CreBC in Escherichia coli
Defining the growth conditions and promoter-proximal DNA sequences required for activation of gene expression by CreBC in Escherichia coli
复制标题
DOI：
10.1128/jb.00108-08
复制
发表时间：
2008-06-01
影响因子：
3.2
通讯作者：
Avison, Matthew B.
中科院分区：
文献类型：
--
作者：
Cariss, S. James L.;Tayler, Amy E.;Avison, Matthew B.
CreBC是一个双组分系统，控制大肠杆菌中编码多种功能的许多基因（称为ere调节子）的表达，包括中间代谢酶。使用一个报告构建体，我们已经表明，ere调节子基因的表达被激活，在基本培养基中生长时，糖酵解碳源被发酵。当发酵产物被用作碳源时，它也在有氧生长过程中被激活。CreB和CreC对于ere调节子基因表达的激活是必不可少的，但作为creABCD基因簇的一部分编码的CreA和CreD不是。CreB在体外与TTCACnnnnnTTCAC直接重复序列（ere标签）结合，该序列与ere调节子基因启动子相关，是体内基因表达控制所必需的。这些观察结果支持了CreBC是一个功能性双组分系统的假设，该系统参与了E. coli中，证实CreB是一个DNA结合转录调节因子。
CreBC is a two-component system that controls the expression of a number of genes in Escherichia coli (called the ere regulon) that encode diverse functions, including intermediary metabolic enzymes. Using a reporter construct, we have shown that ere regulon gene expression is activated during growth in minimal media when glycollytic carbon sources are being fermented. It also is activated during aerobic growth when fermentation products are being used as carbon sources. CreB and CreC are essential for the activation of ere regulon gene expression, but CreA and CreD, encoded as part of the creABCD gene cluster, are not. CreB binds to a TTCACnnnnnnTTCAC direct repeat (the ere tag) in vitro, and this sequence, which is associated with ere regulon gene promoters, is required for the control of gene expression in vivo. These observations support the hypothesis that CreBC is a functional two-component system involved in the metabolic control of transcription in E. coli and confirm that CreB is a DNA binding transcriptional regulator.