QUANTITATIVE MONITORING OF GENE-EXPRESSION PATTERNS WITH A COMPLEMENTARY-DNA MICROARRAY
QUANTITATIVE MONITORING OF GENE-EXPRESSION PATTERNS WITH A COMPLEMENTARY-DNA MICROARRAY
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DOI：
10.1126/science.270.5235.467
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发表时间：
1995-10-20
期刊：
影响因子：
56.9
通讯作者：
BROWN, PO
中科院分区：
文献类型：
--
作者：
SCHENA, M;SHALON, D;BROWN, PO
开发了一种高容量系统来并行监测多个基因的表达。利用高速机器人在玻璃上打印互补dna制备的微阵列进行相应基因的定量表达测量。由于阵列的小格式和高密度，可以使用2微升的杂交体积来检测来自2微克总细胞信使RNA的探针混合物中的稀有转录本。采用同步双色荧光杂交技术对45个拟南芥基因进行差异表达测定。
A high-capacity system was developed to monitor the expression of many genes in parallel. Microarrays prepared by high-speed robotic printing of complementary DNAs on glass were used for quantitative expression measurements of the corresponding genes. Because of the small format and high density of the arrays, hybridization volumes of 2 microliters could be used that enabled detection of rare transcripts in probe mixtures derived from 2 micrograms of total cellular messenger RNA. Differential expression measurements of 45 Arabidopsis genes were made by means of simultaneous, two-color fluorescence hybridization.