The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis
The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis
复制标题
Cep63旁系同源物Deup1能够实现脊椎动物多纤毛发生的大规模从头中心粒生物发生
DOI：
10.1038/ncb2880
复制
发表时间：
2013-12-01
影响因子：
21.3
通讯作者：
Zhu, Xueliang
中科院分区：
文献类型：
--
作者：
Zhao, Huijie;Zhu, Lei;Zhu, Xueliang
在高等脊椎动物中，密集的多纤毛对于管腔流动和去除厚粘液是重要的。为了产生数百个用于多纤毛发生的基底体，特化的终末分化的上皮细胞经历大量的中心粒扩增。然而，在增殖细胞中，中心粒复制在每个细胞周期仅发生一次。细胞如何确保在不同的情况下适当调节中心粒生物发生知之甚少。我们报告，中心粒扩增是由两个重复的基因，Cep63和Deup1。Cep63调节母中心粒依赖性中心粒复制。Deup1调控后体组装，介导大规模从头中心粒生物发生。与Cep63类似，Deup1与Cep152结合，然后招募Plk4来激活中心粒生物合成。系统发育分析表明，Deup1在脊椎动物进化过程中从某个叶鳍鱼类祖先的Cep63中分化出来，随后被四足动物所采用。因此，Cep63基因的重复使得中心粒重复机器的不依赖于母亲中心粒的组装能够满足对中心粒数目的不同要求。
Dense multicilia in higher vertebrates are important for luminal flow and the removal of thick mucus. To generate hundreds of basal bodies for multiciliogenesis, specialized terminally differentiated epithelial cells undergo massive centriole amplification. In proliferating cells, however, centriole duplication occurs only once per cell cycle. How cells ensure proper regulation of centriole biogenesis in different contexts is poorly understood. We report that the centriole amplification is controlled by two duplicated genes, Cep63 and Deup1. Cep63 regulates mother-centriole-dependent centriole duplication. Deup1 governs deuterosome assembly to mediate large-scale de novo centriole biogenesis. Similarly to Cep63, Deup1 binds to Cep152 and then recruits Plk4 to activate centriole biogenesis. Phylogenetic analyses suggest that Deup1 diverged from Cep63 in a certain ancestor of lobe-finned fishes during vertebrate evolution and was subsequently adopted by tetrapods. Thus, the Cep63 gene duplication has enabled mother-centriole-independent assembly of the centriole duplication machinery to satisfy different requirements for centriole number.