GABAergic neurons in the olfactory cortex projecting to the lateral hypothalamus in mice
GABAergic neurons in the olfactory cortex projecting to the lateral hypothalamus in mice
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DOI：
10.1038/s41598-019-43580-1
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发表时间：
2019-05-09
影响因子：
4.6
通讯作者：
Manabe, Hiroyuki
中科院分区：
文献类型：
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作者：
Murata, Koshi;Kinoshita, Tomoki;Manabe, Hiroyuki
嗅觉引导目标导向的行为，包括进食。为了研究中枢嗅觉神经回路如何控制小鼠的进食行为，我们从一个重要的进食中心——外侧下丘脑（LH）进行了逆行追踪。我们观察到一群逆行标记的细胞分布在嗅柄的后腹侧区域。组织化学分析显示，大多数逆行标记的投射神经元表达谷氨酸脱羧酶65/67 (GAD65/67)，但不表达谷氨酸囊泡转运蛋白1 （VGIuT1）。我们将这个包含gaba能投射神经元的区域命名为腹侧嗅核（VON），以区别于传统的嗅脚。与嗅觉结节和伏隔核中的神经元相比，VON神经元对纹状体神经元标志物DARPP-32的免疫反应较低，这是VON与腹侧纹状体的区别。荧光标记证实了VON神经元和嗅球投射神经元之间的突触接触。狂犬病病毒介导的跨突触标记显示，VON神经元接受来自嗅球、其他嗅皮质、对角带水平肢和前额皮质的突触输入。总的来说，这些结果确定了嗅觉皮层中新的gaba能投射神经元，这些神经元可能整合嗅觉感觉和自上而下的输入，并向LH发送抑制输出，这可能调节气味引导的LH相关行为。
Olfaction guides goal-directed behaviours including feeding. To investigate how central olfactory neural circuits control feeding behaviour in mice, we performed retrograde tracing from the lateral hypothalamus (LH), an important feeding centre. We observed a cluster of retrogradely labelled cells distributed in the posteroventral region of the olfactory peduncle. Histochemical analyses revealed that the majority of these retrogradely labelled projection neurons expressed glutamic acid decarboxylase 65/67 (GAD65/67), but not vesicular glutamate transporter 1 (VGIuT1). We named this region containing GABAergic projection neurons the ventral olfactory nucleus (VON) to differentiate it from the conventional olfactory peduncle. VON neurons were less immunoreactive for DARPP-32, a striatal neuron marker, compared to neurons in the olfactory tubercle and nucleus accumbens, which distinguished the VON from the ventral striatum. Fluorescent labelling confirmed putative synaptic contacts between VON neurons and olfactory bulb projection neurons. Rabies-virus-mediated trans-synaptic labelling revealed that VON neurons received synaptic inputs from the olfactory bulb, other olfactory cortices, horizontal limb of the diagonal band, and prefrontal cortex. Collectively, these results identify novel GABAergic projection neurons in the olfactory cortex that may integrate olfactory sensory and top-down inputs and send inhibitory output to the LH, which may modulate odour-guided LH-related behaviours.