Diversity study of nitrifying bacteria in full-scale municipal wastewater treatment plants
Diversity study of nitrifying bacteria in full-scale municipal wastewater treatment plants
复制标题
DOI：
10.1016/j.watres.2006.11.050
复制
发表时间：
2007-03-01
期刊：
影响因子：
12.8
通讯作者：
Rittmann, Bruce E.
中科院分区：
文献类型：
--
作者：
Siripong, Slil;Rittmann, Bruce E.
我们假设，活性污泥法具有稳定和完整的硝化有显着的和类似的多样性和功能冗余之间的氨和亚硝酸盐氧化细菌，尽管在温度，固体停留时间（SRT），和其他操作条件的差异。为了评估这一假设，我们研究了硝化细菌群落的多样性，在所有7个水再生厂（WRP）大芝加哥大都会水再生区（MWRDGC）。这些工厂在进水废水流的类型、工厂规模、水温和污泥龄方面各不相同。本研究利用末端限制性片段长度多态性（T-RFLP）技术，对16 S rRNA基因和组特异性氨单加氧酶功能基因（amoA）进行了研究。我们表明，硝化细菌进行相同的代谢共存于所有WRP研究。我们发现氨氧化细菌（AOB）属于亚硝化单胞菌europaea/eutropha，少养亚硝化单胞菌，亚硝化单胞菌communis，和亚硝化螺旋体谱系在所有的植物。我们还观察到共存的亚硝酸盐氧化细菌（NOB）的硝化杆菌和硝化螺菌属。在WRP之间变化的因素中，只有季节性温度变化似乎改变了硝化群落，特别是亚硝化螺菌和亚硝化单胞菌之间的平衡，尽管两者在冬季和夏季样品中共存。各种硝化菌在所有WRP中的共存是功能冗余的证据，这一特征可能有助于维持硝化系统的稳定性。(C)2007爱思唯尔有限公司保留所有权利。
We hypothesize that activated-sludge processes having stable and complete nitrification have significant and similar diversity and functional redundancy among its ammonia- and nitrite-oxidizing bacteria, despite differences in temperature, solids retention time (SRT), and other operating conditions. To evaluate this hypothesis, we examined the diversity of nitrifying bacterial communities in all seven water-reclamation plants (WRPs) operated by Metropolitan Water Reclamation District of Greater Chicago (MWRDGC). These plants vary in types of influent waste stream, plant size, water temperature, and SRT. We used terminal restriction fragment length polymorphism (T-RFLP) targeting the 16S rRNA gene and group-specific ammonia-monooxygenase functional gene (amoA) to investigate these hard-to-culture nitrifying bacteria in the full-scale WRPs. We demonstrate that nitrifying bacteria carrying out the same metabolism coexist in all WRPs studied. We found ammonia oxidizing bacteria (AOB) belonging to the Nitrosomonas europaea/eutropha, Nitrosomonas oligotropha, Nitrosomonas communis, and Nitrosospira lineages in all plants. We also observed coexisting Nitrobacter and Nitrospira genera for nitrite-oxidizing bacteria (NOB). Among the factors that varied among the WRPs, only the seasonal temperature variation seemed to change the nitrifying community, especially the balance between Nitrosospira and Nitrosomonas, although both coexisted in winter and summer samples. The coexistence of various nitrifiers in all WRPs is evidence of functional redundancy, a feature that may help maintain the stability of the system for nitrification. (C) 2007 Elsevier Ltd. All rights reserved.