Hydrogen sulfide interacts with calcium signaling to enhance the chromium tolerance in Setaria italica
Hydrogen sulfide interacts with calcium signaling to enhance the chromium tolerance in Setaria italica
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硫化氢与钙信号相互作用，增强谷子的铬耐受性
DOI：
10.1016/j.ceca.2014.10.004
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发表时间：
2014-12-01
期刊：
影响因子：
4
通讯作者：
Pei, Yanxi
中科院分区：
文献类型：
--
作者：
Fang, Huihui;Jing, Tao;Pei, Yanxi
细胞内Ca ~（2+）浓度的振荡是植物许多生物学过程中的主要事件，包括胁迫反应。硫化氢（H2S）是一种新兴的气体递质，通过与Ca 2+信号相互作用，在植物对铬（Cr 6+）胁迫的响应中发挥积极作用。虽然Ca 2+在介导生物和非生物胁迫中与H2S相似，但这两种途径之间的串扰仍不清楚。在本研究中，Ca 2+信号与H2S相互作用，产生一个复杂的生理反应，这增强了Cr 6+在谷子（Setaria italica）的耐受性。结果表明，Cr 6+胁迫激活了内源性H2S合成以及Ca 2+信号传导。此外，由Cr 6+胁迫引起的毒性症状被50 μ M H2S和20 mM Ca 2+强烈缓和。相反，治疗与H2S合成抑制剂和Ca 2+螯合剂之前，Cr 6+暴露加重这些毒性症状。有趣的是，Ca 2+上调表达的两个重要的金属代谢因子，MT 3A和PCS，参与重金属螯合剂的生物合成，在H2S依赖的方式来科普Cr 6+胁迫。这些结果也表明，H2S依赖的途径是一个组成部分的Ca 2+激活的抗氧化系统和H2S部分贡献Ca 2+激活的抗氧化系统。(C)2014爱思唯尔有限公司版权所有。
The oscillation of intracellular calcium (Ca2+) concentration is a primary event in numerous biological processes in plants, including stress response. Hydrogen sulfide (H2S), an emerging gasotransmitter, was found to have positive effects in plants responding to chromium (Cr6+) stress through interacting with Ca2+ signaling. While Ca2+ resemblances H2S in mediating biotic and abiotic stresses, crosstalk between the two pathways remains unclear. In this study, Ca2+ signaling interacted with H2S to produce a complex physiological response, which enhanced the Cr6+ tolerance in foxtail millet (Setaria italica). Results indicate that Cr6+ stress activated endogenous H2S synthesis as well as Ca2+ signaling. Moreover, toxic symptoms caused by Cr6+ stress were strongly moderated by 50 mu M H2S and 20 mM Ca2+. Conversely, treatments with H2S synthesis inhibitor and Ca2+ chelators prior to Cr6+-exposure aggravated these toxic symptoms. Interestingly, Ca2+ upregulated expression of two important factors in metal metabolism, MT3A and PCS, which participated in the biosynthesis of heavy metal chelators, in a H2S-dependent manner to cope with Cr6+ stress. These findings also suggest that the H2S dependent pathway is a component of the Ca2+ activating antioxidant system and H2S partially contributes Ca2+-activating antioxidant system. (C) 2014 Elsevier Ltd. All rights reserved.