Circadian rhythms in isolated brain regions
Circadian rhythms in isolated brain regions
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DOI：
10.1523/jneurosci.22-01-00350.2002
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发表时间：
2002-01-01
影响因子：
5.3
通讯作者：
Block, GD
中科院分区：
文献类型：
--
作者：
Abe, M;Herzog, ED;Block, GD
哺乳动物下丘脑的视交叉上核（SCN）被认为是驱动行为和生理日常节律的主昼夜节律起搏器。然而，有证据表明存在超SCN昼夜节律振荡器。从携带Per-luciferase转基因的大鼠中培养的神经组织用于监测不同脑区的固有Per 1表达模式及其对光周期变化的反应。尽管许多表达Per的脑区在培养中是节律性的，但在27个被检查的脑区中有14个是节律性的。松果体和脑垂体都表达了在体外持续>3 d的节律，在主观夜间表达达到峰值。嗅球和腹侧下丘脑的核团表达节律性，在夜间达到峰值，而其他脑区的节律性较弱，在夜间达到峰值，或者是节律性的。在光周期提前或延迟6小时后，松果体、下丘脑室旁核和弓状核各自以不同的动力学调整其节律性的相位。总之，这些结果表明，大脑包含多个，阻尼昼夜振荡器以外的SCN。这些振荡器彼此的相位可能在协调大脑活动及其对光周期变化的调整中发挥关键作用。
The suprachiasmatic nucleus (SCN) of the mammalian hypothalamus has been referred to as the master circadian pacemaker that drives daily rhythms in behavior and physiology. There is, however, evidence for extra-SCN circadian oscillators. Neural tissues cultured from rats carrying the Per-luciferase transgene were used to monitor the intrinsic Per1 expression patterns in different brain areas and their response to changes in the light cycle. Although many Per- expressing brain areas were arrhythmic in culture, 14 of the 27 areas examined were rhythmic. The pineal and pituitary glands both expressed rhythms that persisted for >3 d in vitro, with peak expression during the subjective night. Nuclei in the olfactory bulb and the ventral hypothalamus expressed rhythmicity with peak expression at night, whereas other brain areas were either weakly rhythmic and peaked at night, or arrhythmic. After a 6 hr advance or delay in the light cycle, the pineal, paraventricular nucleus of the hypothalamus, and arcuate nucleus each adjusted the phase of their rhythmicity with different kinetics. Together, these results indicate that the brain contains multiple, damped circadian oscillators outside the SCN. The phasing of these oscillators to one another may play a critical role in coordinating brain activity and its adjustment to changes in the light cycle.