Expression, Splicing, and Evolution of the Myosin Gene Family in Plants
Expression, Splicing, and Evolution of the Myosin Gene Family in Plants
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DOI：
10.1104/pp.110.170720
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发表时间：
2011-03-01
期刊：
影响因子：
7.4
通讯作者：
Dolja, Valerian V.
中科院分区：
文献类型：
--
作者：
Peremyslov, Valera V.;Mockler, Todd C.;Dolja, Valerian V.
植物有两种肌球蛋白，VIII和XI。肌球蛋白XI参与细胞器运输、丝状肌动蛋白组织以及细胞和植物生长。由于肌球蛋白基因家族的庞大规模，这些分子马达的知识仍然是零散的。利用深度转录组测序和生物信息学，我们系统地研究了两种模式植物，拟南芥（Arabidopsis thaliana）和短柄草（Brachypodium distachyon）的肌球蛋白基因。我们改进了肌球蛋白基因模型，发现肌球蛋白基因经历选择性剪接。我们实验验证了拟南芥肌球蛋白XI-K的基因模型，它在细胞内部动力学中起着主要作用，以及其Brachypodium直系同源物。我们发现，通过XI-K基因的部分复制而出现的被称为HDK（肌球蛋白XI-K的无头衍生物）的拟南芥基因受到发育调控。在短柄草中也发现了一个结构相似的基因。我们的分析揭示了两种主要的肌球蛋白基因表达模式，即花粉/雄蕊特异性和无处不在的整个植物的表达。我们还发现，几个肌球蛋白XI可以有节奏地表达。系统发育重建表明，被子植物的最后共同祖先拥有两个肌球蛋白VIII和五个肌球蛋白XI，其中许多经历了额外的谱系特异性复制。
Plants possess two myosin classes, VIII and XI. The myosins XI are implicated in organelle transport, filamentous actin organization, and cell and plant growth. Due to the large size of myosin gene families, knowledge of these molecular motors remains patchy. Using deep transcriptome sequencing and bioinformatics, we systematically investigated myosin genes in two model plants, Arabidopsis (Arabidopsis thaliana) and Brachypodium (Brachypodium distachyon). We improved myosin gene models and found that myosin genes undergo alternative splicing. We experimentally validated the gene models for Arabidopsis myosin XI-K, which plays the principal role in cell interior dynamics, as well as for its Brachypodium ortholog. We showed that the Arabidopsis gene dubbed HDK (for headless derivative of myosin XI-K), which emerged through a partial duplication of the XI-K gene, is developmentally regulated. A gene with similar architecture was also found in Brachypodium. Our analyses revealed two predominant patterns of myosin gene expression, namely pollen/stamen-specific and ubiquitous expression throughout the plant. We also found that several myosins XI can be rhythmically expressed. Phylogenetic reconstructions indicate that the last common ancestor of the angiosperms possessed two myosins VIII and five myosins XI, many of which underwent additional lineage-specific duplications.