Expression of the human antiapoptotic protein Bcl-2 increases nerolidol production in engineered yeast
Expression of the human antiapoptotic protein Bcl-2 increases nerolidol production in engineered yeast
复制标题
人类抗凋亡蛋白 Bcl-2 的表达可增加工程酵母中橙花叔醇的产量
DOI：
10.1016/j.procbio.2022.05.009
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发表时间：
2022-05
影响因子：
4.4
通讯作者：
Wei Yuan
中科院分区：
文献类型：
--
作者：
Jie Sun;Xiancheng Wang;Kechen Yu;Yu Zang;Zhenzhen Qu;Chun Wei;Wei Yuan
Mammalian B-cell lymphoma/leukemia 2 (Bcl-2) has cytoprotective effects against oxidative stress in yeast cells. This study showed that human Bcl-2 increased nerolidol production in an engineered Saccharomyces cerevisiae strain with higher cell viability and lower reactive oxygen species levels. Our previous study found that the overexpression of HAC1 , the key regulator of endoplasmic reticulum stress, increased the nerolidol titer levels. Endoplasmic reticulum stress has crosstalk with oxidative stress. We thus investigated the coincident effect of the overexpression of BCL-2 and HAC1 , and found that both of them changed the transcription of genes involved in the fatty acid metabolism pathway and resulted in increased nerolidol synthesis. The addition of 3 mM linoleic acid further increased the nerolidol titer to 725.5 mg/L. Finally, fed-batch fermentation was implemented and resulted in the accumulation of 15.5 g/L nerolidol. This study provides a simple and effective strategy for sesquiterpene production in engineered yeast. • Anti-apoptotic protein Bcl-2 improves nerolidol production in engineered yeast. • BCL-2 overexpression regulated the metabolism on transcriptional level. • Linoleic acid addition increased the nerolidol titer of the BCL-2 overexpressing strain.
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影响因子：
3.5
作者：
Palmieri, L;Lasorsa, FM;Walker, JE
通讯作者：
Walker, JE
DOI：
--
发表时间：
2016
期刊：
--
影响因子：
--
作者：
Zengyi Shao;Hua Zhao;Huimin Zhao
通讯作者：
Zengyi Shao;Hua Zhao;Huimin Zhao
影响因子：
3.4
作者：
Qu, Zhenzhen;Zhang, Lili;Sun, Jie
通讯作者：
Sun, Jie
DOI：
10.1016/s0021-9258(19)40701-1
发表时间：
1975-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Walenga;W. Lands
通讯作者：
R. Walenga;W. Lands
影响因子：
--
作者：
Iwamoto, MA;Fairclough, SR;Engebrecht, J
通讯作者：
Engebrecht, J