Release of glucagon like peptide-1 (GLP-1) by carbohydrates in the perfused rat ileum
Release of glucagon like peptide-1 (GLP-1) by carbohydrates in the perfused rat ileum
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DOI：
10.1007/s005920050059
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发表时间：
1997-03-01
影响因子：
3.8
通讯作者：
Ramadori, G
中科院分区：
文献类型：
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作者：
Ritzel, U;Fromme, A;Ramadori, G
在体内灌流大鼠回肠中研究了各种碳水化合物对胰高血糖素样肽-1（GLP-1）释放的影响。在用钠/葡萄糖协同转运蛋白（D-葡萄糖、D-半乳糖、甲基-α-D-葡萄糖苷和3-O-甲基-D-葡萄糖）的底物进行管腔灌注后，肠系膜静脉流出物中的GLP-1浓度显著增加。D-果糖诱导GLP-1的钠非依赖性释放。碳水化合物，如2-脱氧-D-葡萄糖和N-乙酰-D-葡糖胺，不是管腔钠/葡萄糖或果糖转运蛋白的底物，不影响GLP-1的释放。由于甲基-α D-葡萄糖苷不是基底外侧葡萄糖转运机制的底物，并且3-O-甲基-D-葡萄糖在肠细胞内不代谢，因此可以得出结论，碳水化合物的细胞内代谢和细胞内清除对于诱导大鼠GLP-1分泌并不重要。
The effect of various carbohydrates on glucagon-like peptide-1 (GLP-1) release was studied in the in vivo perfused rat ileum. GLP-1 concentrations in the mesenteric venous effluent increased significantly after luminal perfusion with substrates of a sodium/glucose cotransporter (D-glucose, D-galactose, methyl-alpha D-glucoside, and 3-O-methyl-D-glucose). D-Fructose induced a sodium-independent release of GLP-1. Carbohydrates like 2-deoxy-D-glucose and N-acetyl-D-glucosamine, which are not substrates of a luminal sodium/glucose or fructose transporter, did not affect GLP-1 release. Since methyl-alpha D-glucoside is not a substrate of the basolateral glucose transport mechanism and 3-O-methyl-D-glucose is not metabolized within intestinal cells, it is concluded that intracellular metabolism of carbohydrates and intracellular removal are not essential to induce GLP-1 secretion in rats.